Mitochondrial protein import: nucleoside triphosphates are involved in conferring import-competence to precursors - PubMed (original) (raw)
Mitochondrial protein import: nucleoside triphosphates are involved in conferring import-competence to precursors
N Pfanner et al. Cell. 1987.
Abstract
The role of nucleoside triphosphates (NTPs) in mitochondrial protein import was investigated with the precursors of N. crassa ADP/ATP carrier, F1-ATPase subunit beta, F0-ATPase subunit 9, and fusion proteins between subunit 9 and mouse dihydrofolate reductase. NTPs were necessary for the initial interaction of precursors with the mitochondria and for the completion of translocation of precursors from the mitochondrial surface into the mitochondria. Higher levels of NTPs were required for the latter reactions as compared with the early stages of import. Import of precursors having identical presequences but different mature protein parts required different levels of NTPs. The sensitivity of precursors in reticulocyte lysate to proteases was decreased by removal of NTPs and increased by their readdition. We suggest that the hydrolysis of NTPs is involved in modulating the folding state of precursors in the cytosol, thereby conferring import competence.
Similar articles
- Mitochondrial precursor proteins are imported through a hydrophilic membrane environment.
Pfanner N, Hartl FU, Guiard B, Neupert W. Pfanner N, et al. Eur J Biochem. 1987 Dec 1;169(2):289-93. doi: 10.1111/j.1432-1033.1987.tb13610.x. Eur J Biochem. 1987. PMID: 2891506 - Proteinaceous receptors for the import of mitochondrial precursor proteins.
Zwizinski C, Schleyer M, Neupert W. Zwizinski C, et al. J Biol Chem. 1984 Jun 25;259(12):7850-6. J Biol Chem. 1984. PMID: 6330086 - The yeast mitochondrial protein import receptor Mas20p binds precursor proteins through electrostatic interaction with the positively charged presequence.
Haucke V, Lithgow T, Rospert S, Hahne K, Schatz G. Haucke V, et al. J Biol Chem. 1995 Mar 10;270(10):5565-70. doi: 10.1074/jbc.270.10.5565. J Biol Chem. 1995. PMID: 7890675 - How mitochondria import proteins.
Hay R, Böhni P, Gasser S. Hay R, et al. Biochim Biophys Acta. 1984 Jan 27;779(1):65-87. doi: 10.1016/0304-4157(84)90004-2. Biochim Biophys Acta. 1984. PMID: 6318829 Review. No abstract available. - Mitochondrial protein import in animals.
Mori M, Terada K. Mori M, et al. Biochim Biophys Acta. 1998 May 27;1403(1):12-27. doi: 10.1016/s0167-4889(98)00021-4. Biochim Biophys Acta. 1998. PMID: 9622585 Review. No abstract available.
Cited by
- Structure and function of the S. pombe III-IV-cyt c supercomplex.
Moe A, Dimogkioka AR, Rapaport D, Öjemyr LN, Brzezinski P. Moe A, et al. Proc Natl Acad Sci U S A. 2023 Nov 14;120(46):e2307697120. doi: 10.1073/pnas.2307697120. Epub 2023 Nov 8. Proc Natl Acad Sci U S A. 2023. PMID: 37939086 Free PMC article. - Mechanisms of mitochondrial respiratory adaptation.
Bennett CF, Latorre-Muro P, Puigserver P. Bennett CF, et al. Nat Rev Mol Cell Biol. 2022 Dec;23(12):817-835. doi: 10.1038/s41580-022-00506-6. Epub 2022 Jul 8. Nat Rev Mol Cell Biol. 2022. PMID: 35804199 Free PMC article. Review. - The mitochondrial protein Sideroflexin 3 (SFXN3) influences neurodegeneration pathways in vivo.
Ledahawsky LM, Terzenidou ME, Edwards R, Kline RA, Graham LC, Eaton SL, van der Hoorn D, Chaytow H, Huang YT, Groen EJN, Motyl AAL, Lamont DJ, Tokatlidis K, Wishart TM, Gillingwater TH. Ledahawsky LM, et al. FEBS J. 2022 Jul;289(13):3894-3914. doi: 10.1111/febs.16377. Epub 2022 Feb 6. FEBS J. 2022. PMID: 35092170 Free PMC article. - Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Morgenstern M, Peikert CD, Lübbert P, Suppanz I, Klemm C, Alka O, Steiert C, Naumenko N, Schendzielorz A, Melchionda L, Mühlhäuser WWD, Knapp B, Busch JD, Stiller SB, Dannenmaier S, Lindau C, Licheva M, Eickhorst C, Galbusera R, Zerbes RM, Ryan MT, Kraft C, Kozjak-Pavlovic V, Drepper F, Dennerlein S, Oeljeklaus S, Pfanner N, Wiedemann N, Warscheid B. Morgenstern M, et al. Cell Metab. 2021 Dec 7;33(12):2464-2483.e18. doi: 10.1016/j.cmet.2021.11.001. Epub 2021 Nov 19. Cell Metab. 2021. PMID: 34800366 Free PMC article. - Neuroprotective Potential of Mild Uncoupling in Mitochondria. Pros and Cons.
Zorov DB, Andrianova NV, Babenko VA, Pevzner IB, Popkov VA, Zorov SD, Zorova LD, Plotnikov EY, Sukhikh GT, Silachev DN. Zorov DB, et al. Brain Sci. 2021 Aug 8;11(8):1050. doi: 10.3390/brainsci11081050. Brain Sci. 2021. PMID: 34439669 Free PMC article. Review.