cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase - PubMed (original) (raw)
Comparative Study
cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase
B W Cherney et al. Proc Natl Acad Sci U S A. 1987 Dec.
Abstract
Recently we described a full-length cDNA for the human nuclear enzyme poly(ADP-ribose) polymerase. Here, we report the chromosomal localization and partial map of the human gene for this enzyme as well as the complete coding sequence for this protein. The nucleotide sequence reveals a single 3042-base open reading frame encoding a protein with a predicted Mr of 113,135. A comparison of this deduced amino acid sequence with the amino acid sequence of three peptides derived from human poly(ADP-ribose) polymerase revealed a match of 27 amino acid residues. A computer-derived structural analysis of the enzyme and a search for similarities with other proteins confirmed that the polymerase belongs to a subfamily of DNA/NAD-binding proteins and DNA-repair proteins. Possible Zn2+-binding "fingers," a nucleotide-binding fold, and a nuclear transport signal were noted. Additionally, chromosomal mapping has identified polymerase-hybridizing sequences on human chromosomes 1 (the active gene), 13, and 14 (processed pseudogenes). Using the polymerase cDNA as a probe, we also have detected several DNA restriction fragment length polymorphisms in normal humans.
References
- J Biol Chem. 1980 Nov 10;255(21):10502-8 - PubMed
- J Biol Chem. 1979 May 10;254(9):3647-51 - PubMed
- Eur J Biochem. 1981 Sep;119(1):23-9 - PubMed
- Microbiol Rev. 1983 Mar;47(1):1-45 - PubMed
- Anal Biochem. 1983 Oct 15;134(2):347-54 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases