Isolation and sequence of the promoter region of the human multidrug-resistance (P-glycoprotein) gene - PubMed (original) (raw)

. 1987 Dec 25;262(36):17432-6.

Affiliations

Free article

Isolation and sequence of the promoter region of the human multidrug-resistance (P-glycoprotein) gene

K Ueda et al. J Biol Chem. 1987.

Free article

Abstract

Intrinsic and acquired multidrug resistance is an important problem in cancer therapy. Multidrug resistance results from overexpression of the MDR 1 gene, which encodes a drug-efflux pump called P-glycoprotein. We have isolated a 1-kilobase genomic fragment containing the major transcription initiation sites for the human MDR 1 gene. Ribonuclease protection experiments using this fragment indicate that normal human adrenal, colon, and liver cells, the human hepatoma cell line HepG2, and vinblastine-selected human KB multidrug-resistant cells initiate transcription of the MDR 1 gene at the same site within this fragment. The 0.43-kilobase region upstream from the major transcription initiation site linked to the chloramphenicol acetyltransferase gene showed promoter activity in CV-1 monkey kidney cells and in human KB cells. The putative promoter region has a consensus CAAT box and two GC box-like sequences, but no TATA sequence. This identification and isolation of promoter sequences for the MDR 1 gene will permit studies on how expression of this gene is regulated in normal human tissues and cancers.

PubMed Disclaimer

Similar articles

Cited by

MeSH terms

Substances

LinkOut - more resources