Molecular cloning and sequencing of a cDNA encoding the thioesterase domain of the rat fatty acid synthetase - PubMed (original) (raw)
. 1988 Jan 25;263(3):1146-50.
Affiliations
- PMID: 2891707
Free article
Molecular cloning and sequencing of a cDNA encoding the thioesterase domain of the rat fatty acid synthetase
J Naggert et al. J Biol Chem. 1988.
Free article
Abstract
A cloned cDNA containing the entire coding sequence for the long-chain S-acyl fatty acid synthetase thioester hydrolase (thioesterase I) component as well as the 3'-noncoding region of the fatty acid synthetase has been isolated using an expression vector and domain-specific antibodies. The coding region was assigned to the thioesterase I domain by identification of sequences coding for characterized peptide fragments, amino-terminal analysis of the isolated thioesterase I domain and the presence of the serine esterase active-site sequence motif. The thioesterase I domain is 306 amino acids long with a calculated molecular mass of 33,476 daltons; its DNA is flanked at the 5'-end by a region coding for the acyl carrier protein domain and at the 3'-end by a 1,537-base pairs-long noncoding sequence with a poly(A) tail. The thioesterase I domain exhibits a low, albeit discernible, homology with the discrete medium-chain S-acyl fatty acid synthetase thioester hydrolases (thioesterase II) from rat mammary gland and duck uropygial gland, suggesting a distant but common evolutionary ancestry for these proteins.
Similar articles
- Cloning and sequencing of the cDNA for S-acyl fatty acid synthase thioesterase from the uropygial gland of mallard duck.
Poulose AJ, Rogers L, Cheesbrough TM, Kolattukudy PE. Poulose AJ, et al. J Biol Chem. 1985 Dec 15;260(29):15953-8. J Biol Chem. 1985. PMID: 2415525 - Cloning and sequencing of the medium-chain S-acyl fatty acid synthetase thioester hydrolase cDNA from rat mammary gland.
Naggert J, Williams B, Cashman DP, Smith S. Naggert J, et al. Biochem J. 1987 Apr 15;243(2):597-601. doi: 10.1042/bj2430597. Biochem J. 1987. PMID: 3632637 Free PMC article. - Amino acid sequence of the serine active-site region of the medium-chain S-acyl fatty acid synthetase thioester hydrolase from rat mammary gland.
Randhawa ZI, Naggert J, Blacher RW, Smith S. Randhawa ZI, et al. Eur J Biochem. 1987 Feb 2;162(3):577-81. doi: 10.1111/j.1432-1033.1987.tb10678.x. Eur J Biochem. 1987. PMID: 3104035 - Complete amino acid sequence of the medium-chain S-acyl fatty acid synthetase thio ester hydrolase from rat mammary gland.
Randhawa ZI, Smith S. Randhawa ZI, et al. Biochemistry. 1987 Mar 10;26(5):1365-73. doi: 10.1021/bi00379a024. Biochemistry. 1987. PMID: 3567174 - Molecular cloning and sequence analysis of complementary DNA encoding rat mammary gland medium-chain S-acyl fatty acid synthetase thio ester hydrolase.
Safford R, de Silva J, Lucas C, Windust JH, Shedden J, James CM, Sidebottom CM, Slabas AR, Tombs MP, Hughes SG. Safford R, et al. Biochemistry. 1987 Mar 10;26(5):1358-64. doi: 10.1021/bi00379a023. Biochemistry. 1987. PMID: 3105579
Cited by
- Expression in Escherichia coli, purification and characterization of two mammalian thioesterases involved in fatty acid synthesis.
Naggert J, Witkowski A, Wessa B, Smith S. Naggert J, et al. Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):787-90. doi: 10.1042/bj2730787. Biochem J. 1991. PMID: 1671746 Free PMC article. - Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase.
Amy CM, Witkowski A, Naggert J, Williams B, Randhawa Z, Smith S. Amy CM, et al. Proc Natl Acad Sci U S A. 1989 May;86(9):3114-8. doi: 10.1073/pnas.86.9.3114. Proc Natl Acad Sci U S A. 1989. PMID: 2717611 Free PMC article. - Sequence pattern for the occurrence of N-glycosylation in proteins.
Yan B, Zhang W, Ding J, Gao P. Yan B, et al. J Protein Chem. 1999 Jul;18(5):511-21. doi: 10.1023/a:1020643015113. J Protein Chem. 1999. PMID: 10524769 - BFIT, a unique acyl-CoA thioesterase induced in thermogenic brown adipose tissue: cloning, organization of the human gene and assessment of a potential link to obesity.
Adams SH, Chui C, Schilbach SL, Yu XX, Goddard AD, Grimaldi JC, Lee J, Dowd P, Colman S, Lewin DA. Adams SH, et al. Biochem J. 2001 Nov 15;360(Pt 1):135-42. doi: 10.1042/bj3600135. Biochem J. 2001. PMID: 11696000 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases