CD3-negative lymphokine-activated cytotoxic cells express the CD3 epsilon gene - PubMed (original) (raw)
. 1988 Mar 1;140(5):1685-9.
Affiliations
- PMID: 2894394
CD3-negative lymphokine-activated cytotoxic cells express the CD3 epsilon gene
R Biassoni et al. J Immunol. 1988.
Abstract
The expression of genes encoding different polypeptide chains of the TCR-CD3 complex was analyzed in a panel of cloned MHC-unrestricted cytotoxic cells. The clones were derived from CD3+ and CD3- human PBL. After expansion in rIL-2, all clones were able to lyse the NK-sensitive target cell line K562. In contrast, lysis of fresh tumor cells was achieved almost exclusively by CD3- clones. To test whether a known TCR-CD3 complex may be involved in MHC-unrestricted cytotoxicity, total RNA from nine CD3+ and 11 CD3- clones was isolated and hybridized with DNA probes for the TCR alpha-, beta-, and gamma-chains and for the CD3 gamma-, delta-, and epsilon-chains. TCR gamma transcripts were present at high levels in CD3+CD4- CD8- clones but were undetectable in all CD3- clones. Lysis of fresh tumor cells is an activity which can be independent of the TCR alpha beta and TCR gamma complexes because the CD3- clones did not express these TCR genes. Interestingly, all CD3- clones expressed CD3 epsilon transcripts, but not CD3 gamma- or delta-transcripts. CD3- lymphokine-activated cytotoxic cells may therefore be derived from immature T cells which do not yet express a complete CD3 complex. The CD3 epsilon chain, if expressed in CD3- cells in association with other molecules, could be involved in the activation and lytic function of these MHC-unrestricted cytotoxic cells.
Similar articles
- Antigen specific and MHC nonrestricted cytotoxicity of T cell receptor alpha beta+ and gamma delta+ human T cell clones isolated in IL-4.
Paliard X, Yssel H, Blanchard D, Waitz JA, deVries JE, Spits H. Paliard X, et al. J Immunol. 1989 Jul 15;143(2):452-7. J Immunol. 1989. PMID: 2472439 - Molecular and cellular analysis of human T lymphocytes expressing gamma delta T-cell receptor.
Moretta L, Ciccone E, Ferrini S, Pelicci PG, Mingari MC, Zeromski J, Bottino C, Grossi C, Moretta A. Moretta L, et al. Immunol Rev. 1991 Apr;120:117-35. doi: 10.1111/j.1600-065x.1991.tb00590.x. Immunol Rev. 1991. PMID: 1650757 Review.
Cited by
- In vitro expansion of CD3/TCR- human thymocyte populations that selectively lack CD3 delta gene expression: a phenotypic and functional analysis.
Poggi A, Biassoni R, Pella N, Paolieri F, Bellomo R, Bertolini A, Moretta L, Mingari MC. Poggi A, et al. J Exp Med. 1990 Nov 1;172(5):1409-18. doi: 10.1084/jem.172.5.1409. J Exp Med. 1990. PMID: 2146363 Free PMC article. - Long-term cultures of human peripheral blood lymphocytes with recombinant human interleukin-2 generate a population of virtually pure CD3+ CD16- CD56- large granular lymphocyte LAK cells.
Roussel E, Gerrard JM, Greenberg AH. Roussel E, et al. Clin Exp Immunol. 1990 Nov;82(2):416-21. doi: 10.1111/j.1365-2249.1990.tb05463.x. Clin Exp Immunol. 1990. PMID: 1700746 Free PMC article. - Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin.
Kohn LA, Hao QL, Sasidharan R, Parekh C, Ge S, Zhu Y, Mikkola HK, Crooks GM. Kohn LA, et al. Nat Immunol. 2012 Oct;13(10):963-71. doi: 10.1038/ni.2405. Epub 2012 Sep 2. Nat Immunol. 2012. PMID: 22941246 Free PMC article. - An unusual form of alternative splicing in the HLA-DNA gene.
Rosen-Bronson S, Long EO. Rosen-Bronson S, et al. Immunogenetics. 1991;33(2):124-31. doi: 10.1007/BF00210826. Immunogenetics. 1991. PMID: 1999352 - Tumour necrosis factor-alpha induces an increase in susceptibility of human glioblastoma U87-MG cells to natural killer cell-mediated lysis.
Kondo S, Yin D, Takeuchi J, Morimura T, Miyatake SI, Nakatsu S, Oda Y, Kikuchi H. Kondo S, et al. Br J Cancer. 1994 Apr;69(4):627-32. doi: 10.1038/bjc.1994.123. Br J Cancer. 1994. PMID: 7908214 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials