Development and application of a bioluminescent imaging mouse model for Chikungunya virus based on pseudovirus system - PubMed (original) (raw)
. 2017 Nov 7;35(47):6387-6394.
doi: 10.1016/j.vaccine.2017.10.007. Epub 2017 Oct 12.
Affiliations
- PMID: 29031692
- DOI: 10.1016/j.vaccine.2017.10.007
Development and application of a bioluminescent imaging mouse model for Chikungunya virus based on pseudovirus system
Jiajing Wu et al. Vaccine. 2017.
Abstract
Chikungunya virus (CHIKV) is an arthropod-borne virus that is transmitted to humans primarily via the bite of an infected mosquito. Infection of humans by CHIKV can cause chikungunya fever which is an acute febrile illness associated with severe, often debilitating polyarthralgias. Since a re-emergence of CHIKV in 2004, the virus has spread into novel locations in nearly 40 countries including non-endemic regions and has led to millions of cases of disease throughout countries. Handling of CHIKV is restricted to the high-containment Biosafety Level 3 (BSL-3) facilities, which greatly impede the research progress of this virus. In this study, an envelope-pseudotyped virus expressing the firefly luciferase reporter protein (pHIV-CHIKV-Fluc) was generated. An in vitro sensitive neutralizing assay and an in vivo bioluminescent-imaging-based mouse infection model had been developed based on the CHIKV pseudovirus. Utilizing the platform, protection effect of DNA vaccine was evaluated. Therefore, this study provides a safe, sensitive and visualizing model for evaluating vaccines and antiviral therapies against CHIKV in low containment BSL-2 laboratories.
Keywords: Animal model; Bioluminescent imaging; Chikungunya virus; DNA vaccine; Pseudovirus.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Similar articles
- Development of a neutralization assay based on the pseudotyped chikungunya virus of a Korean isolate.
Chung WC, Hwang KY, Kang SJ, Kim JO, Song MJ. Chung WC, et al. J Microbiol. 2020 Jan;58(1):46-53. doi: 10.1007/s12275-020-9384-0. Epub 2019 Nov 25. J Microbiol. 2020. PMID: 31768937 Free PMC article. - Liposomal Delivery of the RNA Genome of a Live-Attenuated Chikungunya Virus Vaccine Candidate Provides Local, but Not Systemic Protection After One Dose.
Abeyratne E, Tharmarajah K, Freitas JR, Mostafavi H, Mahalingam S, Zaid A, Zaman M, Taylor A. Abeyratne E, et al. Front Immunol. 2020 Mar 5;11:304. doi: 10.3389/fimmu.2020.00304. eCollection 2020. Front Immunol. 2020. PMID: 32194557 Free PMC article. - Prime-boost immunization strategies against Chikungunya virus.
Hallengärd D, Lum FM, Kümmerer BM, Lulla A, Lulla V, García-Arriaza J, Fazakerley JK, Roques P, Le Grand R, Merits A, Ng LF, Esteban M, Liljeström P. Hallengärd D, et al. J Virol. 2014 Nov;88(22):13333-43. doi: 10.1128/JVI.01926-14. Epub 2014 Sep 10. J Virol. 2014. PMID: 25210177 Free PMC article. - Pseudotyped Viruses for the Alphavirus Chikungunya Virus.
Wu J, Huang W, Wang Y. Wu J, et al. Adv Exp Med Biol. 2023;1407:299-312. doi: 10.1007/978-981-99-0113-5_16. Adv Exp Med Biol. 2023. PMID: 36920704 Review. - Regulatory considerations in development of vaccines to prevent disease caused by Chikungunya virus.
Yang S, Fink D, Hulse A, Pratt RD. Yang S, et al. Vaccine. 2017 Sep 5;35(37):4851-4858. doi: 10.1016/j.vaccine.2017.07.065. Epub 2017 Jul 29. Vaccine. 2017. PMID: 28760614 Review.
Cited by
- Establishment and validation of a pseudovirus neutralization assay for SARS-CoV-2.
Nie J, Li Q, Wu J, Zhao C, Hao H, Liu H, Zhang L, Nie L, Qin H, Wang M, Lu Q, Li X, Sun Q, Liu J, Fan C, Huang W, Xu M, Wang Y. Nie J, et al. Emerg Microbes Infect. 2020 Dec;9(1):680-686. doi: 10.1080/22221751.2020.1743767. Emerg Microbes Infect. 2020. PMID: 32207377 Free PMC article. - Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2.
Balinsky C, Jani V, Sun P, Williams M, Defang G, Porter KR. Balinsky C, et al. Methods Mol Biol. 2022;2452:361-378. doi: 10.1007/978-1-0716-2111-0_21. Methods Mol Biol. 2022. PMID: 35554917 - Natural products as a source of Coronavirus entry inhibitors.
Szabó D, Crowe A, Mamotte C, Strappe P. Szabó D, et al. Front Cell Infect Microbiol. 2024 Feb 21;14:1353971. doi: 10.3389/fcimb.2024.1353971. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38449827 Free PMC article. Review. - Application of pseudovirus system in the development of vaccine, antiviral-drugs, and neutralizing antibodies.
Xiang Q, Li L, Wu J, Tian M, Fu Y. Xiang Q, et al. Microbiol Res. 2022 May;258:126993. doi: 10.1016/j.micres.2022.126993. Epub 2022 Feb 16. Microbiol Res. 2022. PMID: 35240544 Free PMC article. Review. - Construction and immunogenicity of an mRNA vaccine against chikungunya virus.
Liu J, Lu X, Li X, Huang W, Fang E, Li W, Liu X, Liu M, Li J, Li M, Zhang Z, Song H, Ying B, Li Y. Liu J, et al. Front Immunol. 2023 Mar 15;14:1129118. doi: 10.3389/fimmu.2023.1129118. eCollection 2023. Front Immunol. 2023. PMID: 37006310 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical