Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes - PubMed (original) (raw)

Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes

S Froshauer et al. J Cell Biol. 1988 Dec.

Abstract

Using morphological and cell biological techniques, we have shown that the RNA replicase of Semliki Forest and Sindbis virus (two closely related alphaviruses) is located in complex ribonucleoprotein structures associated with the cytoplasmic surface of modified secondary lysosomes and endosomes. These nucleoprotein complexes often form a bridge between the membrane of the endocytic vacuole and the rough endoplasmic reticulum where the synthesis of the structural proteins of these viruses occurs. The results suggest that these cytopathic vacuoles constitute sites not only for viral RNA synthesis, but also for translation of structural proteins, and for the assembly of nucleocapsids.

PubMed Disclaimer

Similar articles

• Partially Uncleaved Alphavirus Replicase Forms Spherule Structures in the Presence and Absence of RNA Template.
  Hellström K, Kallio K, Utt A, Quirin T, Jokitalo E, Merits A, Ahola T. Hellström K, et al. J Virol. 2017 Aug 24;91(18):e00787-17. doi: 10.1128/JVI.00787-17. Print 2017 Sep 15. J Virol. 2017. PMID: 28701392 Free PMC article.
• Purification of Highly Active Alphavirus Replication Complexes Demonstrates Altered Fractionation of Multiple Cellular Membranes.
  Pietilä MK, van Hemert MJ, Ahola T. Pietilä MK, et al. J Virol. 2018 Mar 28;92(8):e01852-17. doi: 10.1128/JVI.01852-17. Print 2018 Apr 15. J Virol. 2018. PMID: 29367248 Free PMC article.
• The alphavirus replicase protein nsP1 is membrane-associated and has affinity to endocytic organelles.
  Peränen J, Laakkonen P, Hyvönen M, Kääriäinen L. Peränen J, et al. Virology. 1995 Apr 20;208(2):610-20. doi: 10.1006/viro.1995.1192. Virology. 1995. PMID: 7747433
• Viral RNA replication in association with cellular membranes.
  Salonen A, Ahola T, Kääriäinen L. Salonen A, et al. Curr Top Microbiol Immunol. 2005;285:139-73. doi: 10.1007/3-540-26764-6_5. Curr Top Microbiol Immunol. 2005. PMID: 15609503 Free PMC article. Review.
• Alphavirus polymerase and RNA replication.
  Pietilä MK, Hellström K, Ahola T. Pietilä MK, et al. Virus Res. 2017 Apr 15;234:44-57. doi: 10.1016/j.virusres.2017.01.007. Epub 2017 Jan 16. Virus Res. 2017. PMID: 28104453 Review.

Cited by

• The molecular dissection of TRIM25's RNA-binding mechanism provides key insights into its antiviral activity.
  Álvarez L, Haubrich K, Iselin L, Gillioz L, Ruscica V, Lapouge K, Augsten S, Huppertz I, Choudhury NR, Simon B, Masiewicz P, Lethier M, Cusack S, Rittinger K, Gabel F, Leitner A, Michlewski G, Hentze MW, Allain FHT, Castello A, Hennig J. Álvarez L, et al. Nat Commun. 2024 Oct 1;15(1):8485. doi: 10.1038/s41467-024-52918-x. Nat Commun. 2024. PMID: 39353916 Free PMC article.
• Evolution of RNA Viruses: Reasons for the Existence of Separate Plus, Minus, and Double-Strand Replication Strategies.
  Park H, Higgs PG. Park H, et al. Viruses. 2024 Jul 5;16(7):1081. doi: 10.3390/v16071081. Viruses. 2024. PMID: 39066243 Free PMC article.
• In situ fate of Chikungunya virus replication organelles.
  Girard J, Le Bihan O, Lai-Kee-Him J, Girleanu M, Bernard E, Castellarin C, Chee M, Neyret A, Spehner D, Holy X, Favier A-L, Briant L, Bron P. Girard J, et al. J Virol. 2024 Jul 23;98(7):e0036824. doi: 10.1128/jvi.00368-24. Epub 2024 Jun 28. J Virol. 2024. PMID: 38940586
• Chikungunya virus infection in human microglial C20 cells induces mitochondria-mediated apoptosis.
  Kumar N, Santhoshkumar R, Venkataswamy MM. Kumar N, et al. Front Cell Infect Microbiol. 2024 Apr 23;14:1380736. doi: 10.3389/fcimb.2024.1380736. eCollection 2024. Front Cell Infect Microbiol. 2024. PMID: 38716191 Free PMC article.
• Trans-Amplifying RNA: A Journey from Alphavirus Research to Future Vaccines.
  Yıldız A, Răileanu C, Beissert T. Yıldız A, et al. Viruses. 2024 Mar 25;16(4):503. doi: 10.3390/v16040503. Viruses. 2024. PMID: 38675846 Free PMC article. Review.

References

1. 1. Virology. 1967 May;32(1):128-43 - PubMed
2. 1. J Virol. 1968 Nov;2(11):1326-38 - PubMed
3. 1. Int Rev Cytol. 1969;26:235-302 - PubMed
4. 1. Ann Med Exp Biol Fenn. 1969;47(4):235-48 - PubMed
5. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database