Identification of specific binding proteins for a nuclear location sequence - PubMed (original) (raw)
. 1989 Jan 19;337(6204):276-9.
doi: 10.1038/337276a0.
Affiliations
- PMID: 2911368
- DOI: 10.1038/337276a0
Identification of specific binding proteins for a nuclear location sequence
S A Adam et al. Nature. 1989.
Abstract
The nuclear envelope is a selective barrier against the movement of macromolecules between the nucleus and cytoplasm. Nuclear proteins larger than relative molecular mass 20,000-40,000 are probably actively transported across the envelope through the nuclear pore complex and are directed by specific nuclear location sequences (NLS) in the proteins. NLS mediate the nuclear import of isolated nuclear proteins after microinjection into whole cells and the nuclear accumulation of chimaeric proteins or of non-nuclear proteins conjugated to synthetic peptides. The best-characterized NLS is the simian virus 40 large T-antigen sequence. We have identified two proteins of rat liver by chemical cross-linking that interact with a synthetic peptide containing this sequence: this interaction is specific for a functional NLS, is saturable, and high affinity. The binding proteins are present in a post-mitochondrial supernatant, in nuclei and in a nuclear envelope fraction, which is consistent with a role in the transport of nuclear proteins from the cytoplasm to the nucleus.
Similar articles
- Inhibition of nuclear protein import by a monoclonal antibody against a novel class of nuclear pore proteins.
Pandey S, Karande AA, Mishra K, Parnaik VK. Pandey S, et al. Exp Cell Res. 1994 Jun;212(2):243-54. doi: 10.1006/excr.1994.1140. Exp Cell Res. 1994. PMID: 7514536 - Interaction of a nuclear location signal with isolated nuclear envelopes and identification of signal-binding proteins by photoaffinity labeling.
Benditt JO, Meyer C, Fasold H, Barnard FC, Riedel N. Benditt JO, et al. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9327-31. doi: 10.1073/pnas.86.23.9327. Proc Natl Acad Sci U S A. 1989. PMID: 2556708 Free PMC article. - Mediators of nuclear protein import target karyophilic proteins to pore complexes of cytoplasmic annulate lamellae.
Cordes VC, Rackwitz HR, Reidenbach S. Cordes VC, et al. Exp Cell Res. 1997 Dec 15;237(2):419-33. doi: 10.1006/excr.1997.3806. Exp Cell Res. 1997. PMID: 9434638 - Transport across the nuclear envelope: enigmas and explanations.
Dingwall C. Dingwall C. Bioessays. 1991 May;13(5):213-8. doi: 10.1002/bies.950130503. Bioessays. 1991. PMID: 1654049 Review. - Active transport of proteins into the nucleus.
Wagner P, Kunz J, Koller A, Hall MN. Wagner P, et al. FEBS Lett. 1990 Nov 26;275(1-2):1-5. doi: 10.1016/0014-5793(90)81425-n. FEBS Lett. 1990. PMID: 2175710 Review.
Cited by
- Alternative splicing introduces a nuclear localization signal that targets multifunctional CaM kinase to the nucleus.
Srinivasan M, Edman CF, Schulman H. Srinivasan M, et al. J Cell Biol. 1994 Aug;126(4):839-52. doi: 10.1083/jcb.126.4.839. J Cell Biol. 1994. PMID: 7519621 Free PMC article. - Design of bacteriophage T4-based artificial viral vectors for human genome remodeling.
Zhu J, Batra H, Ananthaswamy N, Mahalingam M, Tao P, Wu X, Guo W, Fokine A, Rao VB. Zhu J, et al. Nat Commun. 2023 May 30;14(1):2928. doi: 10.1038/s41467-023-38364-1. Nat Commun. 2023. PMID: 37253769 Free PMC article. - Visualization of transport-related configurations of the nuclear pore transporter.
Akey CW. Akey CW. Biophys J. 1990 Aug;58(2):341-55. doi: 10.1016/S0006-3495(90)82381-X. Biophys J. 1990. PMID: 2207242 Free PMC article. - Chromosome condensation caused by loss of RCC1 function requires the cdc25C protein that is located in the cytoplasm.
Seki T, Yamashita K, Nishitani H, Takagi T, Russell P, Nishimoto T. Seki T, et al. Mol Biol Cell. 1992 Dec;3(12):1373-88. doi: 10.1091/mbc.3.12.1373. Mol Biol Cell. 1992. PMID: 1337289 Free PMC article. - The rate of nuclear cytoplasmic protein transport is determined by the casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen.
Rihs HP, Jans DA, Fan H, Peters R. Rihs HP, et al. EMBO J. 1991 Mar;10(3):633-9. doi: 10.1002/j.1460-2075.1991.tb07991.x. EMBO J. 1991. PMID: 1848177 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources