5' flanking and first intron sequences of the human beta-actin gene required for efficient promoter activity - PubMed (original) (raw)
5' flanking and first intron sequences of the human beta-actin gene required for efficient promoter activity
R M Frederickson et al. Nucleic Acids Res. 1989.
Free PMC article
Abstract
We have identified a CCAAT box element that is required for the efficient transcription of the human beta-actin gene. Both in vivo transient transfection assays in cultured HeLa cells and in vitro run-off transcription assays in HeLa whole cell extracts demonstrated the requirement of this element for efficient promoter activity. A gel mobility shift assay revealed a Hela nuclear factor that specifically interacted with the beta-actin CCAAT element in vitro; mutation of the first three base pairs of the CCAAT pentanucleotide abolished binding of this factor. Competition gel shift experiments revealed that three sequence elements located within the beta-actin promoter, each containing a CC(A/T)6GG motif similar to that contained within the c-fos serum response element, were able to bind a different nuclear factor, serum response factor (SRF). One of these CC(A/T)6GG motifs is contained within a first intron fragment that enhanced transcription from a heterologous promoter in vivo.
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References
- Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 - PubMed
- Cell. 1981 Dec;27(2 Pt 1):299-308 - PubMed
- Proc Natl Acad Sci U S A. 1982 Nov;79(21):6438-42 - PubMed
- Nucleic Acids Res. 1983 Mar 25;11(6):1759-71 - PubMed
- Proc Natl Acad Sci U S A. 1983 Jun;80(11):3183-7 - PubMed
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