Cell cycle perturbation and cell death after exposure of a human lymphoblastoid cell strain to N-methyl-N'-nitro-N-nitrosoguanidine - PubMed (original) (raw)

Cell cycle perturbation and cell death after exposure of a human lymphoblastoid cell strain to N-methyl-N'-nitro-N-nitrosoguanidine

K A Black et al. Am J Pathol. 1989 Jan.

Abstract

The mechanisms involved in cell death caused by carcinogens that methylate DNA are poorly understood. In this study, the cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was studied in exponentially growing T5-1 human lymphoblastoid cells. MNNG exposure killed cells and inhibited proliferation of the remaining viable cells. Reduction in cell viability, which coincided with the accumulation of cells in the late S phase of the cell cycle, was not apparent until the population had completed one doubling. Fluorescence-activated cell sorting of fluorescein diacetate-stained, MNNG-treated cells into live and dead subpopulations revealed that all cycle phases were well represented in the live fraction, whereas the dead fraction consisted primarily of cells with a sub-G1 DNA content. Thus, cell death after MNNG exposure occurred during the second cell cycle after treatment apparently as a consequence of perturbation of DNA replication and the degradation of nuclear DNA.

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References

1. Mutat Res. 1987 Apr;177(2):267-76 - PubMed
1. Proc Natl Acad Sci U S A. 1982 Feb;79(4):1235-9 - PubMed
1. J Cell Sci Suppl. 1987;6:333-53 - PubMed
1. Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41 - PubMed
1. Mutat Res. 1970 Sep;10(3):237-46 - PubMed

Cell cycle perturbation and cell death after exposure of a human lymphoblastoid cell strain to N-methyl-N'-nitro-N-nitrosoguanidine - PubMed (original) (raw)