Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation - PubMed (original) (raw)
Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation
R P Ricciardi et al. Proc Natl Acad Sci U S A. 1979 Oct.
Abstract
We describe a simple procedure for isolating specific mRNAs and for mapping them to the regions of the DNA from which they originate. The method involves hybridization of total cytoplasmic RNA to restriction fragments of DNA that have been fractionated in agarose gels and immobilized on nitrocellulose filters. The hybridization-selected RNAs are eluted and translated in a cell-free system in order to identify their encoded polypeptides. Optimal hybridization and filter washing conditions are given for selection of mRNAs from adenovirus 2-infected cells and transformed cells.
Similar articles
- Purification of specific adenovirus 2 RNAs by preparative hybridization and selective thermal elution.
McGrogan M, Spector DJ, Goldenberg CJ, Halbert D, Raskas HJ. McGrogan M, et al. Nucleic Acids Res. 1979 Feb;6(2):593-607. doi: 10.1093/nar/6.2.583. Nucleic Acids Res. 1979. PMID: 424306 Free PMC article. - Cell-free translation of highly purified adenovirus messenger RNA.
Eron L, Westphal H. Eron L, et al. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3385-9. doi: 10.1073/pnas.71.9.3385. Proc Natl Acad Sci U S A. 1974. PMID: 4530310 Free PMC article. - Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
Lewis JB, Atkins JF, Anderson CW, Baum PR, Gesteland RF. Lewis JB, et al. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344-8. doi: 10.1073/pnas.72.4.1344. Proc Natl Acad Sci U S A. 1975. PMID: 1093178 Free PMC article. - The isolation of eukaryotic messenger RNA.
Taylor JM. Taylor JM. Annu Rev Biochem. 1979;48:681-717. doi: 10.1146/annurev.bi.48.070179.003341. Annu Rev Biochem. 1979. PMID: 382995 Review. No abstract available. - Mammalian messenger RNA.
Mathews MB. Mathews MB. Essays Biochem. 1973;9:59-102. Essays Biochem. 1973. PMID: 4589417 Review. No abstract available.
Cited by
- Characterization of the gene for mp20: a Drosophila muscle protein that is not found in asynchronous oscillatory flight muscle.
Ayme-Southgate A, Lasko P, French C, Pardue ML. Ayme-Southgate A, et al. J Cell Biol. 1989 Feb;108(2):521-31. doi: 10.1083/jcb.108.2.521. J Cell Biol. 1989. PMID: 2537318 Free PMC article. - Putative amino acid sequence of chick calcium-binding protein deduced from a complementary DNA sequence.
Wilson PW, Harding M, Lawson DE. Wilson PW, et al. Nucleic Acids Res. 1985 Dec 20;13(24):8867-81. doi: 10.1093/nar/13.24.8867. Nucleic Acids Res. 1985. PMID: 3841205 Free PMC article. - Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation.
Cohen G, Fessl F, Traczyk A, Rytka J, Ruis H. Cohen G, et al. Mol Gen Genet. 1985;200(1):74-9. doi: 10.1007/BF00383315. Mol Gen Genet. 1985. PMID: 3897793 - Sequence of cDNA for rat cystathionine gamma-lyase and comparison of deduced amino acid sequence with related Escherichia coli enzymes.
Erickson PF, Maxwell IH, Su LJ, Baumann M, Glode LM. Erickson PF, et al. Biochem J. 1990 Jul 15;269(2):335-40. doi: 10.1042/bj2690335. Biochem J. 1990. PMID: 2201285 Free PMC article. - An adenovirus mRNA which encodes a 14,700-Mr protein that maps to the last open reading frame of region E3 is expressed during infection.
Wang EW, Scott MO, Ricciardi RP. Wang EW, et al. J Virol. 1988 Apr;62(4):1456-9. doi: 10.1128/JVI.62.4.1456-1459.1988. J Virol. 1988. PMID: 2831407 Free PMC article.
References
- Nucleic Acids Res. 1978 Jun;5(6):2039-53 - PubMed
- Nucleic Acids Res. 1979 Jun 11;6(7):2483-97 - PubMed
- Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 - PubMed
- Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 - PubMed
- Proc Natl Acad Sci U S A. 1977 Oct;74(10):4370-4 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources