Rapid activation by interferon alpha of a latent DNA-binding protein present in the cytoplasm of untreated cells - PubMed (original) (raw)
Rapid activation by interferon alpha of a latent DNA-binding protein present in the cytoplasm of untreated cells
T C Dale et al. Proc Natl Acad Sci U S A. 1989 Feb.
Abstract
The highly conserved interferon (IFN)-stimulated regulatory elements of the human genes 6-16 and 9-27 bind to one or more proteins (E factor) detected in extracts of human Bristol 8 B cells or human foreskin fibroblast cells treated with IFN-alpha. E factor is not detectable in extracts of untreated cells and appears in IFN-treated cells within less than 1 min in a form extractable with low salt and thus presumably not bound to DNA. After a few more minutes, the level of this form decreases in parallel with the increase of a form extractable only with high salt and thus presumably bound to DNA. Induction of E factor by IFN-alpha can occur in nuclei-free cytoplasts, whereas no E factor was detected in IFN-treated nucleoplasts. Together, these results suggest a model for signal transduction in which latent E factor, located in the cytoplasm, is activated or released from an inhibitor very rapidly upon binding of IFN-alpha to its receptor. Active E factor can then migrate to the nucleus, where it binds to the IFN-stimulated regulatory elements of IFN-regulated genes, activating their transcription.
Similar articles
- Cytoplasmic activation of ISGF3, the positive regulator of interferon-alpha-stimulated transcription, reconstituted in vitro.
Levy DE, Kessler DS, Pine R, Darnell JE Jr. Levy DE, et al. Genes Dev. 1989 Sep;3(9):1362-71. doi: 10.1101/gad.3.9.1362. Genes Dev. 1989. PMID: 2606351 - The interferon-inducible Stat2:Stat1 heterodimer preferentially binds in vitro to a consensus element found in the promoters of a subset of interferon-stimulated genes.
Ghislain JJ, Wong T, Nguyen M, Fish EN. Ghislain JJ, et al. J Interferon Cytokine Res. 2001 Jun;21(6):379-88. doi: 10.1089/107999001750277853. J Interferon Cytokine Res. 2001. PMID: 11440635 - Interferon-gamma regulation of the human mimecan promoter.
Tasheva ES, Conrad GW. Tasheva ES, et al. Mol Vis. 2003 Jun 30;9:277-87. Mol Vis. 2003. PMID: 12835654
Cited by
- Inborn errors of immunity with loss- and gain-of-function germline mutations in STAT1.
Asano T, Utsumi T, Kagawa R, Karakawa S, Okada S. Asano T, et al. Clin Exp Immunol. 2023 Apr 25;212(2):96-106. doi: 10.1093/cei/uxac106. Clin Exp Immunol. 2023. PMID: 36420581 Free PMC article. Review. - STAT1 and Its Crucial Role in the Control of Viral Infections.
Tolomeo M, Cavalli A, Cascio A. Tolomeo M, et al. Int J Mol Sci. 2022 Apr 7;23(8):4095. doi: 10.3390/ijms23084095. Int J Mol Sci. 2022. PMID: 35456913 Free PMC article. Review. - STAT1 gain-of-function heterozygous cell models reveal diverse interferon-signature gene transcriptional responses.
Scott O, Lindsay K, Erwood S, Mollica A, Roifman CM, Cohn RD, Ivakine EA. Scott O, et al. NPJ Genom Med. 2021 May 14;6(1):34. doi: 10.1038/s41525-021-00196-7. NPJ Genom Med. 2021. PMID: 33990617 Free PMC article. - Human STAT1 Gain-of-Function Heterozygous Mutations: Chronic Mucocutaneous Candidiasis and Type I Interferonopathy.
Okada S, Asano T, Moriya K, Boisson-Dupuis S, Kobayashi M, Casanova JL, Puel A. Okada S, et al. J Clin Immunol. 2020 Nov;40(8):1065-1081. doi: 10.1007/s10875-020-00847-x. Epub 2020 Aug 27. J Clin Immunol. 2020. PMID: 32852681 Free PMC article. Review.
References
- Immunol Rev. 1979;47:3-61 - PubMed
- Proc Natl Acad Sci U S A. 1989 Feb;86(3):840-4 - PubMed
- Cell. 1984 Oct;38(3):745-55 - PubMed
- J Biol Chem. 1986 Jan 5;261(1):453-9 - PubMed
- Annu Rev Genet. 1985;19:209-52 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources