Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification - PubMed (original) (raw)
Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification
S Pääbo. Proc Natl Acad Sci U S A. 1989 Mar.
Abstract
Several chemical and enzymatic properties were examined in the DNA extracted from dry remains of soft tissues that vary in age from 4 to 13,000 years and represent four species, including two extinct animals (the marsupial wolf and giant ground sloth). The DNA obtained was invariably of a low average molecular size and damaged by oxidative processes, which primarily manifest themselves as modifications of pyrimidines and sugar residues as well as baseless sites and intermolecular cross-links. This renders molecular cloning difficult. However, the polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance. This opens up the prospect of performing diachronical studies of molecular evolutionary genetics.
Similar articles
- Chloroplast DNA sequence from a miocene Magnolia species.
Golenberg EM, Giannasi DE, Clegg MT, Smiley CJ, Durbin M, Henderson D, Zurawski G. Golenberg EM, et al. Nature. 1990 Apr 12;344(6267):656-8. doi: 10.1038/344656a0. Nature. 1990. PMID: 2325772 - PCR amplification, cloning, and sequencing of ancient DNA.
Fulton TL, Stiller M. Fulton TL, et al. Methods Mol Biol. 2012;840:111-9. doi: 10.1007/978-1-61779-516-9_15. Methods Mol Biol. 2012. PMID: 22237529 - DNA damage promotes jumping between templates during enzymatic amplification.
Pääbo S, Irwin DM, Wilson AC. Pääbo S, et al. J Biol Chem. 1990 Mar 15;265(8):4718-21. J Biol Chem. 1990. PMID: 2307682 - Genetic analyses from ancient DNA.
Pääbo S, Poinar H, Serre D, Jaenicke-Despres V, Hebler J, Rohland N, Kuch M, Krause J, Vigilant L, Hofreiter M. Pääbo S, et al. Annu Rev Genet. 2004;38:645-79. doi: 10.1146/annurev.genet.37.110801.143214. Annu Rev Genet. 2004. PMID: 15568989 Review. - Ancient DNA.
Höss M. Höss M. Horm Res. 1995;43(4):118-20. doi: 10.1159/000184253. Horm Res. 1995. PMID: 7750909 Review.
Cited by
- Patellae as a source of DNA in forensic and archaeological analysis.
Geršak ŽM, Golob A, Kravanja P, Concato M, Leskovar T, Pajnič IZ. Geršak ŽM, et al. Int J Legal Med. 2024 Nov 8. doi: 10.1007/s00414-024-03363-4. Online ahead of print. Int J Legal Med. 2024. PMID: 39514086 - Improved detection of methylation in ancient DNA.
Sawyer S, Gelabert P, Yakir B, Llanos-Lizcano A, Sperduti A, Bondioli L, Cheronet O, Neugebauer-Maresch C, Teschler-Nicola M, Novak M, Pap I, Szikossy I, Hajdu T, Moiseyev V, Gromov A, Zariņa G, Meshorer E, Carmel L, Pinhasi R. Sawyer S, et al. Genome Biol. 2024 Oct 10;25(1):261. doi: 10.1186/s13059-024-03405-5. Genome Biol. 2024. PMID: 39390557 Free PMC article. - soibean: High-Resolution Taxonomic Identification of Ancient Environmental DNA Using Mitochondrial Pangenome Graphs.
Vogel NA, Rubin JD, Pedersen AG, Sackett PW, Pedersen MW, Renaud G. Vogel NA, et al. Mol Biol Evol. 2024 Oct 4;41(10):msae203. doi: 10.1093/molbev/msae203. Mol Biol Evol. 2024. PMID: 39361595 Free PMC article. - Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine.
Yan B, Wang D, Ettwiller L. Yan B, et al. Genome Res. 2024 Jul 23;34(6):904-913. doi: 10.1101/gr.278294.123. Genome Res. 2024. PMID: 38858087 Free PMC article. - Assessing the impact of post-mortem damage and contamination on imputation performance in ancient DNA.
Garrido Marques A, Rubinacci S, Malaspinas AS, Delaneau O, Sousa da Mota B. Garrido Marques A, et al. Sci Rep. 2024 Mar 14;14(1):6227. doi: 10.1038/s41598-024-56584-3. Sci Rep. 2024. PMID: 38486065 Free PMC article.
References
- Adv Food Res. 1965;14:167-283 - PubMed
- J Biol Chem. 1974 Mar 10;249(5):1536-40 - PubMed
- Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed
- J Biol Chem. 1977 May 10;252(9):2808-14 - PubMed
- J Biol Chem. 1977 May 25;252(10):3286-94 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources