Increased Abundance of Clostridium and Fusobacterium in Gastric Microbiota of Patients with Gastric Cancer in Taiwan - PubMed (original) (raw)

Increased Abundance of Clostridium and Fusobacterium in Gastric Microbiota of Patients with Gastric Cancer in Taiwan

Yung-Yu Hsieh et al. Sci Rep. 2018.

Abstract

Helicobacter pylori is recognised as a main risk factor for gastric cancer. However, approximately half of the patients with gastritis are negative for H. pylori infection, and the abundance of H. pylori decreases in patients with cancer. In the current study, we profiled gastric epithelium-associated bacterial species in patients with gastritis, intestinal metaplasia, and gastric cancer to identify additional potential pathogenic bacteria. The overall composition of the microbiota was similar between the patients with gastritis and those with intestinal metaplasia. H. pylori was present in half of the non-cancer group, and the dominant bacterial species in the H. pylori-negative patients were Burkholderia, Enterobacter, and Leclercia. The abundance of those bacteria was similar between the cancer and non-cancer groups, whereas the frequency and abundance of H. pylori were significantly lower in the cancer group. Instead, Clostridium, Fusobacterium, and Lactobacillus species were frequently abundant in patients with gastric cancer, demonstrating a gastric cancer-specific bacterial signature. A receiver operating characteristic curve analysis showed that Clostridium colicanis and Fusobacterium nucleatum exhibited a diagnostic ability for gastric cancer. Our findings indicate that the gastric microenvironment is frequently colonised by Clostridium and Fusobacterium in patients with gastric cancer.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1

Figure 1

Identification of changes in the composition of the microbiota between patients with gastric cancer and those with other gastrointestinal disorders by 16S ribosomal DNA metagenomic analysis. (A) The abundance of Helicobacter pylori in the patients’ microbiota. (B) The proportions of high-abundance in-transit microbes. (C) The abundance of Burkholderia, Enterobacter, and Leclercia. (D) The abundance of Clostridium, Fusobacterium, and Lactobacillus.

Figure 2

Figure 2

Bacteria specifically enriched in the gastric cancer-associated microbiota.

Figure 3

Figure 3

Identification of the bacterial signature of gastric cancer. Multidimensional analyses were performed considering various combinations of the following bacterial species: Clostridium colicanis (Cc), Fusobacterium canifelinum (Fc), Fusobacterium nucleatum (Fn), Lactobacillus gasseri (Lg), Lactobacillus reuteri (Lr), Megasphaera micronuciformis (Mm), Prevotella intermedia (Pi), Prevotella oris (Po), Streptococcus gordonii (Sg), and Streptococcus parasanguinis (Sp).

Figure 4

Figure 4

Evaluation of the discriminatory power of using bacterial abundance as a diagnostic tool. (A) The discriminatory power of the abundance of each individual species C. colicanis, F. canifelinum, F. nucleatum, L. gasseri, and L. reuteri was tested by a receiver operating characteristic curve analysis. (B) The combined abundance of C. colicanis and F. canifelinum (Cc + Fn), C. colicanis, F. canifelinum, and F. nucleatum (Cc + Fc + Fn), and C. colicanis, F. canifelinum, F. nucleatum, Lactobacillus gasseri, and Lactobacillus reuteri (Cc + Fc + Fn + Lg + Lr) was examined.

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