Properties of purified T cell subsets. I. In vitro responses to class I vs. class II H-2 alloantigens - PubMed (original) (raw)

Comparative Study

Properties of purified T cell subsets. I. In vitro responses to class I vs. class II H-2 alloantigens

J Sprent et al. J Exp Med. 1985.

Abstract

In light of the widely accepted view that Ia-restricted L3T4+ T helper cells play a decisive role in controlling the differentiation of Lyt-2+ cells, experiments were designed to examine whether Lyt-2+ cells can respond to antigen in the absence of L3T4+ cells. The results showed that highly purified Lyt-2+ cells gave high primary mixed lymphocyte reactions (MLR) to various class I differences, including both mutant and allelic differences; responses to class II (Ia) differences were generally undetectable with Lyt-2+ cells. The intensity of MLR to class I differences was not affected by addition of anti-L3T4 monoclonal antibodies (mAb) to the cultures or by removing T cells from the stimulator populations. Negative selection experiments showed that Lyt-2+ cells could respond to class I differences across Ia barriers. MLR of purified Lyt-2+ cells peaked on days 3-4 and then fell sharply; background responses with syngeneic stimulators (auto-MLR) were virtually absent. Parallel experiments with purified L3T4+ cells showed that this subset responded in MLR only to class II (Ia) and not class I differences, reached peak responses only on day 6 rather than days 3-4, and often gave high auto-MLR. Within the first 3-4 d of culture, MLR were generally higher with Lyt-2+ cells than L3T4+ cells. Although no evidence could be found that Ia-restricted L3T4+ cells were required for the response of Lyt-2+ cells, presentation of antigen by Ia+ cells appeared to be essential. Thus, responses were ablated by pretreating stimulator cells with anti-Ia mAb plus C'. Significantly the failure of Lyt-2+ cells to respond to anti-Ia plus C'-treated stimulators could not be restored by adding syngeneic spleen cells; addition of IL-2 led to only a minor (15%) restoration of the response. It is suggested that Ia+ cells provide an obligatory second signal required by Lyt-2+ cells.

PubMed Disclaimer

Cited by

Variable capacity of L3T4+ T cells to cause lethal graft-versus-host disease across minor histocompatibility barriers in mice.
Korngold R, Sprent J. Korngold R, et al. J Exp Med. 1987 Jun 1;165(6):1552-64. doi: 10.1084/jem.165.6.1552. J Exp Med. 1987. PMID: 3108446 Free PMC article.
Properties of purified T cell subsets. II. In vivo responses to class I vs. class II H-2 differences.
Sprent J, Schaefer M, Lo D, Korngold R. Sprent J, et al. J Exp Med. 1986 Apr 1;163(4):998-1011. doi: 10.1084/jem.163.4.998. J Exp Med. 1986. PMID: 3512763 Free PMC article.
T cell stimulation in vivo by lipopolysaccharide (LPS).
Tough DF, Sun S, Sprent J. Tough DF, et al. J Exp Med. 1997 Jun 16;185(12):2089-94. doi: 10.1084/jem.185.12.2089. J Exp Med. 1997. PMID: 9182680 Free PMC article.

References

1. Nat New Biol. 1973 Apr 25;242(121):239-41 - PubMed
1. J Immunol. 1985 Jun;134(6):3557-9 - PubMed
1. J Exp Med. 1975 Jun 1;141(6):1376-89 - PubMed
1. J Exp Med. 1975 Jun 1;141(6):1390-9 - PubMed
1. J Exp Med. 1976 Mar 1;143(3):585-600 - PubMed

Properties of purified T cell subsets. I. In vitro responses to class I vs. class II H-2 alloantigens - PubMed (original) (raw)