[ARTICLE WITHDRAWN] MicroRNA-18a Targets IRF2 and CBX7 to Promote Cell Proliferation in Hepatocellular Carcinoma - PubMed (original) (raw)

[ARTICLE WITHDRAWN] MicroRNA-18a Targets IRF2 and CBX7 to Promote Cell Proliferation in Hepatocellular Carcinoma

Zhang Yongyu et al. Oncol Res. 2018.

Retraction in

Abstract

THIS ARTICLE WAS WITHDRAWN BY THE PUBLISHER IN NOVEMBER 2020.

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Figures

Figure 1

Figure 1

Expression of miR-18a in HCC cell lines SK-HEP-1, Hep3B, and Bel-7402 and normal liver cell line HL-7702 (control). ***p < 0.001 versus control. miR-18a, microRNA-18a; HCC, hepatocellular carcinoma.

Figure 2

Figure 2

miR-18a promotes HCC cell proliferation and migration. (A) Expression of miR-18a in the SK-HEP-1 cell line was evaluated after miR-18a mimic, inhibitor, or NC miRNA transfection by RT-qPCR. (B) Expression of miR-18a in the Bel-7402 cell line was evaluated after miR-18a mimic, inhibitor, or NC miRNA transfection by RT-qPCR. (C) Cell proliferation was detected in the SK-HEP-1 cell line after miR-18a mimic, inhibitor, or NC miRNA transfection by MTT assay. (D) Cell proliferation was detected in the Bel-7402 cell line after miR-18a mimic, inhibitor, or NC miRNA transfection by MTT assay. (E) Cell migration was detected in the SK-HEP-1 cell line after miR-18a mimic, inhibitor, or NC miRNA transfection by wound healing assay. (F) Cell migration was detected in the Bel-7402 cell line after miR-18a mimic, inhibitor, or NC miRNA transfection by wound healing assay. ***p < 0.001, **p < 0.01, *p < 0.05. RT-qPCR, quantitative real-time polymerase chain reaction; NC miRNA, negative control microRNA.

Figure 3

Figure 3

IRF2 and CBX7 were the direct targets of miR-18a in HCC. (A) The predicted miR-18a binding sequence in the IRF2 3′-untranslated region (3′-UTR) and the mutated sequence. (B) The predicted miR-18a binding sequence in CBX7 3′-UTR and the mutated sequence. (C) Relative luciferase activity in the Bel-7402 cell line transfected with wild-type IRF2 3′-UTR (WT) or mutated IRF2 3′-UTR (Mut). (D) Relative luciferase activity in the Bel-7402 cell line transfected with CBX73′-UTR WT or CBX73′-UTR Mut. (E) Expression of IRF2 in the Bel-7402 cell line transfected with miR-18a mimic or NC miRNA was examined by Western blot assay. (F) Expression of CBX7 in Bel-7402 cell line transfected with miR-18a mimic or NC miRNA was examined by Western blot assay. **p < 0.01. NC miRNA, negative control microRNA; IRF2, interferon regulatory factor 2; CBX7, chromobox protein homolog 7.

Figure 4

Figure 4

miR-18a promoted HCC cell proliferation and migration by targeting IRF2 and CBX7. (A) Expression of IRF2 was measured by Western blot assay in the Bel-7402 cell line transfected with or without an IRF2 plasmid. (B) Expression of CBX7 was measured by Western blot assay in Bel-7402 cells transfected with or without a CBX7 plasmid. (C) Cell proliferation was measured in the Bel-7402 cell line after miR-18a mimic or IRF2 plasmid transfection by MTT assay. (D) Cell proliferation was measured in the Bel-7402 cell line after miR-18a mimic or CBX7 plasmid transfection using MTT assay. (E) Cell migration was measured in the Bel-7402 cell line after miR-18a mimic or IRF2 plasmid transfection by the wound healing assay. (F) Cell migration was measured in the Bel-7402 cell line after miR-18a mimic or CBX7 plasmid transfection by wound healing assay. ***p < 0.001, **p < 0.01, *p < 0.05.

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