Morphological transformation of established rodent cell lines by high-level expression of the adenovirus type 2 E1a gene - PubMed (original) (raw)
Morphological transformation of established rodent cell lines by high-level expression of the adenovirus type 2 E1a gene
A W Senear et al. Mol Cell Biol. 1986 Apr.
Abstract
When a strong promoter derived from the mouse metallothionein gene was substituted for the homologous adenovirus type 2 E1a promoter, leading to enhanced levels of E1a RNAs and proteins in cells transfected with the chimeric gene, the E1a gene alone was able to induce in established cell lines alterations in cellular morphology and growth properties similar to those produced by the combined action of E1a and E1b genes. The qualitative effects of E1a gene expression upon cellular properties thus depend on the level of expression of the E1a gene. Furthermore, E1a may be the primary transforming gene of adenoviruses, since it produced many of the characteristics of transformed cells that had previously been attributed to E1b.
Similar articles
- Different adenovirus E1A-controlled properties of transformed cells require different levels of E1A expression.
Vaessen RT, Houweling A, van der Eb AJ. Vaessen RT, et al. Gene. 1987;54(2-3):247-54. doi: 10.1016/0378-1119(87)90493-8. Gene. 1987. PMID: 2820846 - Adenovirus early region 3 promoter regulation by E1A/E1B is independent of alterations in DNA binding and gene activation of CREB/ATF and AP1.
Kornuc M, Kliewer S, Garcia J, Harrich D, Li C, Gaynor R. Kornuc M, et al. J Virol. 1990 May;64(5):2004-13. doi: 10.1128/JVI.64.5.2004-2013.1990. J Virol. 1990. PMID: 2139139 Free PMC article. - Functional relatedness between the E1a and E1b regions of group C and group D human adenoviruses.
Jannun R, Chinnadurai G. Jannun R, et al. Virus Res. 1987 Feb;7(1):33-48. doi: 10.1016/0168-1702(87)90056-6. Virus Res. 1987. PMID: 2951934 - Transcriptional and transforming activities of the adenovirus E1A proteins.
Shenk T, Flint J. Shenk T, et al. Adv Cancer Res. 1991;57:47-85. doi: 10.1016/s0065-230x(08)60995-1. Adv Cancer Res. 1991. PMID: 1835254 Review. No abstract available. - Multiple functional domains in the adenovirus E1A gene.
Moran E, Mathews MB. Moran E, et al. Cell. 1987 Jan 30;48(2):177-8. doi: 10.1016/0092-8674(87)90418-1. Cell. 1987. PMID: 2948653 Review. No abstract available.
Cited by
- In vivo potential effects of adenovirus type 5 E1A and E1B on lung carcinogenesis and lymphoproliferative inflammation.
Yang Y, McKerlie C, Lu Z, Wang L, Buchwald M. Yang Y, et al. J Virol. 2008 Aug;82(16):8105-11. doi: 10.1128/JVI.00536-08. Epub 2008 Jun 4. J Virol. 2008. PMID: 18524829 Free PMC article. - The N-terminal region of the adenovirus type 5 E1A proteins can repress expression of cellular genes via two distinct but overlapping domains.
Dorsman JC, Hagmeyer BM, Veenstra J, Elfferich P, Nabben N, Zantema A, van der Eb AJ. Dorsman JC, et al. J Virol. 1995 May;69(5):2962-7. doi: 10.1128/JVI.69.5.2962-2967.1995. J Virol. 1995. PMID: 7707522 Free PMC article. - Different activities of the adenovirus types 5 and 12 E1A regions in transformation with the EJ Ha-ras oncogene.
Jochemsen AG, Bernards R, van Kranen HJ, Houweling A, Bos JL, van der Eb AJ. Jochemsen AG, et al. J Virol. 1986 Sep;59(3):684-91. doi: 10.1128/JVI.59.3.684-691.1986. J Virol. 1986. PMID: 3735492 Free PMC article. - Transforming and mitogenic effects of avian leukemia virus E26 on chicken hematopoietic cells and fibroblasts, respectively, correlate with level of expression of the provirus.
Jurdic P, Benchaibi M, Gandrillon O, Samarut J. Jurdic P, et al. J Virol. 1987 Oct;61(10):3058-65. doi: 10.1128/JVI.61.10.3058-3065.1987. J Virol. 1987. PMID: 3041027 Free PMC article. - Human papillomavirus type 16 DNA cooperates with activated ras in transforming primary cells.
Matlashewski G, Schneider J, Banks L, Jones N, Murray A, Crawford L. Matlashewski G, et al. EMBO J. 1987 Jun;6(6):1741-6. doi: 10.1002/j.1460-2075.1987.tb02426.x. EMBO J. 1987. PMID: 3038534 Free PMC article.
References
- Cell. 1977 Apr;10(4):669-78 - PubMed
- Virology. 1985 Jun;143(2):452-66 - PubMed
- Proc Natl Acad Sci U S A. 1979 Aug;76(8):3665-9 - PubMed
- Methods Enzymol. 1980;65(1):718-49 - PubMed
- Methods Enzymol. 1980;65(1):826-39 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical