Repression of insulin gene expression by adenovirus type 5 E1a proteins - PubMed (original) (raw)

Repression of insulin gene expression by adenovirus type 5 E1a proteins

R W Stein et al. Mol Cell Biol. 1987 Mar.

Abstract

Insulin gene transcription relies on enhancer and promoter elements which are active in pancreatic beta cells. We showed that adenovirus type 5 infection of HIT T-15 cells, a transformed hamster beta cell line, represses insulin gene transcription and mRNA levels. Using expression plasmids transiently introduced into HIT T-15 cells, we showed that adenovirus type 5 E1a transcription regulatory proteins repress insulin enhancer-promoter element activity as assayed with a surrogate xanthine-guanine phosphoribosyltransferase gene. We relate E1a repression of the insulin gene to other examples of repression of enhancer-dependent genes by E1a and discuss the possible relationship of this repression to insulin gene regulation.

PubMed Disclaimer

Similar articles

• Insulin gene enhancer activity is inhibited by adenovirus 5 E1a gene products.
  Stein RW, Whelan J. Stein RW, et al. Mol Cell Biol. 1989 Oct;9(10):4531-4. doi: 10.1128/mcb.9.10.4531-4534.1989. Mol Cell Biol. 1989. PMID: 2531280 Free PMC article.
• Adenovirus E1a proteins repress expression from polyomavirus early and late promoters.
  Velcich A, Kern FG, Basilico C, Ziff EB. Velcich A, et al. Mol Cell Biol. 1986 Nov;6(11):4019-25. doi: 10.1128/mcb.6.11.4019-4025.1986. Mol Cell Biol. 1986. PMID: 3025625 Free PMC article.
• Adenovirus E1a ras cooperation activity is separate from its positive and negative transcription regulatory functions.
  Velcich A, Ziff E. Velcich A, et al. Mol Cell Biol. 1988 May;8(5):2177-83. doi: 10.1128/mcb.8.5.2177-2183.1988. Mol Cell Biol. 1988. PMID: 2968500 Free PMC article.
• Adenovirus promoters and E1A transactivation.
  Berk AJ. Berk AJ. Annu Rev Genet. 1986;20:45-79. doi: 10.1146/annurev.ge.20.120186.000401. Annu Rev Genet. 1986. PMID: 3028247 Review. No abstract available.
• Regulation of early adenovirus gene expression.
  Nevins JR. Nevins JR. Microbiol Rev. 1987 Dec;51(4):419-30. doi: 10.1128/mr.51.4.419-430.1987. Microbiol Rev. 1987. PMID: 2963951 Free PMC article. Review. No abstract available.

Cited by

• E1A-CR3 interaction-dependent and -independent functions of mSur2 in viral replication of early region 1A mutants of mouse adenovirus type 1.
  Fang L, Spindler KR. Fang L, et al. J Virol. 2005 Mar;79(6):3267-76. doi: 10.1128/JVI.79.6.3267-3276.2005. J Virol. 2005. PMID: 15731221 Free PMC article.
• Axl-gas6 interaction counteracts E1A-mediated cell growth suppression and proapoptotic activity.
  Lee WP, Liao Y, Robinson D, Kung HJ, Liu ET, Hung MC. Lee WP, et al. Mol Cell Biol. 1999 Dec;19(12):8075-82. doi: 10.1128/MCB.19.12.8075. Mol Cell Biol. 1999. PMID: 10567533 Free PMC article.
• Mouse adenovirus type 1 early region 1A is dispensable for growth in cultured fibroblasts.
  Ying B, Smith K, Spindler KR. Ying B, et al. J Virol. 1998 Aug;72(8):6325-31. doi: 10.1128/JVI.72.8.6325-6331.1998. J Virol. 1998. PMID: 9658071 Free PMC article.
• The role of mouse adenovirus type 1 early region 1A in acute and persistent infections in mice.
  Smith K, Brown CC, Spindler KR. Smith K, et al. J Virol. 1998 Jul;72(7):5699-706. doi: 10.1128/JVI.72.7.5699-5706.1998. J Virol. 1998. PMID: 9621028 Free PMC article.

References

1. 1. Cell. 1983 Nov;35(1):137-48 - PubMed
2. 1. Mol Cell Biol. 1986 Nov;6(11):4019-25 - PubMed
3. 1. Cell. 1983 Dec;35(3 Pt 2):693-9 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9 - PubMed
5. 1. Diabetes. 1984 Mar;33(3):297-300 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis

• Medical

  • MedlinePlus Health Information