A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter - PubMed (original) (raw)
. 1987 Apr;326(6112):512-5.
doi: 10.1038/326512a0.
- PMID: 2951598
- DOI: 10.1038/326512a0
A TATA box implicated in E1A transcriptional activation of a simple adenovirus 2 promoter
L Wu et al. Nature. 1987 Apr.
Abstract
Adenovirus E1A proteins stimulate transcription by RNA polymerases II and III from many promoters. The detailed mechanism of transcriptional activation (transactivation) by E1A proteins remains unclear, but genetic and biochemical results suggest that E1A products might act to stimulate the activity of cellular transcription factors. In this study, a detailed mutational analysis of the adenovirus E1B promoter was undertaken to define the DNA sequences required for proper basal transcription and E1A transactivation. Two key findings emerged: first the E1B promoter is an unusually simple RNA polymerase II promoter requiring only two sequence elements for proper regulation, the TATA box and a binding site for transcription factor Sp1; and second only mutations in the TATA box interfere with E1A-transactivation, suggesting that E1A mediates its effect on this promoter through the TATA-box transcription factor.
Similar articles
- Multiple transcription factor binding sites mediate adenovirus E1A transactivation.
Pei R, Berk AJ. Pei R, et al. J Virol. 1989 Aug;63(8):3499-506. doi: 10.1128/JVI.63.8.3499-3506.1989. J Virol. 1989. PMID: 2545919 Free PMC article. - Functions of adenovirus E1A.
Berk AJ. Berk AJ. Cancer Surv. 1986;5(2):367-87. Cancer Surv. 1986. PMID: 2946406 - Transactivation of adenovirus E2-early promoter by E1A and E4 6/7 in the context of viral chromosome.
Swaminathan S, Thimmapaya B. Swaminathan S, et al. J Mol Biol. 1996 May 24;258(5):736-46. doi: 10.1006/jmbi.1996.0283. J Mol Biol. 1996. PMID: 8637006 - E1a revisited: the case for multiple cooperative trans-activation domains.
Braithwaite AW, Nelson CC, Bellett AJ. Braithwaite AW, et al. New Biol. 1991 Jan;3(1):18-26. New Biol. 1991. PMID: 1828178 Review. - Adenovirus E1A-dependent trans-activation of transcription.
Nevins JR. Nevins JR. Semin Cancer Biol. 1990 Feb;1(1):59-68. Semin Cancer Biol. 1990. PMID: 2133112 Review.
Cited by
- Identification of G protein-coupled receptor 55 (GPR55) as a target of curcumin.
Harada N, Okuyama M, Teraoka Y, Arahori Y, Shinmori Y, Horiuchi H, Luis PB, Joseph AI, Kitakaze T, Matsumura S, Hira T, Yamamoto N, Iuni T, Goshima N, Schneider C, Inui H, Yamaji R. Harada N, et al. NPJ Sci Food. 2022 Jan 14;6(1):4. doi: 10.1038/s41538-021-00119-x. NPJ Sci Food. 2022. PMID: 35031622 Free PMC article. - Characterization of mouse Bmp5 regulatory injury element in zebrafish wound models.
Heller IS, Guenther CA, Meireles AM, Talbot WS, Kingsley DM. Heller IS, et al. Bone. 2022 Feb;155:116263. doi: 10.1016/j.bone.2021.116263. Epub 2021 Nov 23. Bone. 2022. PMID: 34826632 Free PMC article. - Polycomblike protein PHF1b: a transcriptional sensor for GABA receptor activity.
Saha S, Hu Y, Martin SC, Bandyopadhyay S, Russek SJ, Farb DH. Saha S, et al. BMC Pharmacol Toxicol. 2013 Jul 23;14:37. doi: 10.1186/2050-6511-14-37. BMC Pharmacol Toxicol. 2013. PMID: 23879974 Free PMC article. - Checkpoints in adenoviral production: cross-contamination and E1A.
Haeussler DJ, Evangelista AM, Burgoyne JR, Cohen RA, Bachschmid MM, Pimental DR. Haeussler DJ, et al. PLoS One. 2011;6(8):e23160. doi: 10.1371/journal.pone.0023160. Epub 2011 Aug 3. PLoS One. 2011. PMID: 21826235 Free PMC article. - Profiling the thermodynamic softness of adenoviral promoters.
Choi CH, Rapti Z, Gelev V, Hacker MR, Alexandrov B, Park EJ, Park JS, Horikoshi N, Smerzi A, Rasmussen KØ, Bishop AR, Usheva A. Choi CH, et al. Biophys J. 2008 Jul;95(2):597-608. doi: 10.1529/biophysj.107.123471. Epub 2008 Apr 4. Biophys J. 2008. PMID: 18390611 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical