Silver Nanoparticles Synthesized Using Wild Mushroom Show Potential Antimicrobial Activities against Food Borne Pathogens - PubMed (original) (raw)
Silver Nanoparticles Synthesized Using Wild Mushroom Show Potential Antimicrobial Activities against Food Borne Pathogens
Yugal Kishore Mohanta et al. Molecules. 2018.
Abstract
The present study demonstrates an economical and eco-friendly method for the synthesis of silver nanoparticles (AgNPs) using the wild mushroom Ganoderma sessiliforme. The synthesis of AgNPs was confirmed and the products characterized by UV-visible spectroscopy, dynamic light scattering spectroscopy and X-ray diffraction analysis. Furthermore, Fourier transform infrared spectroscopy (ATR-FTIR) analysis was performed to identify the viable biomolecules involved in the capping and active stabilization of AgNPs. Moreover, the average sizes and morphologies of AgNPs were analyzed by field emission scanning electron microscopy (FE-SEM). The potential impacts of AgNPs on food safety and control were evaluated by the antimicrobial activity of the synthesized AgNPs against common food-borne bacteria, namely, Escherichia coli, Bacillus subtilis, Streptococcus faecalis, Listeria innocua and Micrococcus luteus. The results of this study revealed that the synthesized AgNPs can be used to control the growth of food-borne pathogens and have potential application in the food packaging industry. Moreover, the AgNPs were evaluated for antioxidant activity (aDPPH), for biocompatibility (L-929, normal fibroblast cells), and for cytotoxic effects on human breast adenosarcoma cells (MCF-7 & MDA-MB231) to highlight their potential for use in a variety of bio-applications.
Keywords: Ganoderma sessiliforme; antimicrobial activity; food borne bacteria; silver nanoparticles.
Conflict of interest statement
The authors declare no conflict of interest.
Figures
Figure 1
(A) G. sessiliforme; (B) G. sessiliforme mycelial extract and AgNO3; (C) synthesized silver nanoparticles (AgNPs).
Figure 2
The ultraviolet-visible spectra of silver nanoparticles (AgNPs).
Figure 3
(A) Size distribution of synthesized AgNPs; (B) Zeta potential of synthesized AgNPs by DLS analysis.
Figure 4
ATR-fourier-transformed infrared spectroscopy analysis of silver nanoparticles (AgNPs) and the aqueous extracts of G. sessiliforme.
Figure 5
X–ray diffraction analysis of silver nanoparticles (AgNPs) synthesized by the aqueous extracts of G. sessiliforme.
Figure 6
Morphological characterization through FE–SEM (A) and HR–TEM (B) microscopy of silver nanoparticles (AgNPs) synthesized by the aqueous extracts of G. sessiliforme; (i)Free standing AgNPs observed in HR–TEM; (ii) analysis of grain diameter of a single AgNP in HR–TEM.
Figure 7
Antioxidant potentials of AgNPs and ascorbic acid (DPPH radical scavenging).
Figure 8
Antibacterial activities of AgNPs synthesized by G. sessiliforme (volume-50 µL/well): (A) M. luteus; (B) L. innocua; (C) B. subtilis; (D) S. faecalis; (E) E. coli.
Figure 9
Cytotoxic effect of silver nanoparticles (AgNPs) on L-929 normal fibroblast cell lines.
Figure 10
Cytotoxic effect of silver nanoparticles (AgNPs) on: (A) MCF-7; (B) MDA-MB-231 human breast cancer cells.
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