Coclustering of CD4 (L3T4) molecule with the T-cell receptor is induced by specific direct interaction of helper T cells and antigen-presenting cells - PubMed (original) (raw)
Coclustering of CD4 (L3T4) molecule with the T-cell receptor is induced by specific direct interaction of helper T cells and antigen-presenting cells
A Kupfer et al. Proc Natl Acad Sci U S A. 1987 Aug.
Abstract
Blocking studies with monoclonal antibodies have suggested that helper T cell recognition and triggering involve the CD4 (L3T4) accessory molecule as well as the T-cell receptor (TCR) that is linked to the T3 complex. We have investigated the surface distribution of L3T4 and TCR during the direct interaction of a cloned murine helper T-cell line with an antigen-presenting B-cell line. Using immunofluorescence microscopy, we show that in 1:1 cell couples formed between the two cells, in which a specific interaction can be demonstrated, the L3T4 and the TCR become redistributed on the T-cell surface so that they are concentrated in the cell-cell contact region. This coclustering of L3T4 with TCR occurs only when the relevant antigen and appropriate major histocompatibility class II molecules are presented to the T cell, and it therefore requires the specific interaction of the TCR with its complex ligand on the antigen-presenting cell.
Similar articles
- Anti-L3T4 antibody inhibits the lysis of H-2 class II antigen-negative target cells by L3T4+ cytotoxic T lymphocytes.
Macphail S, Stutman O. Macphail S, et al. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5205-9. doi: 10.1073/pnas.85.14.5205. Proc Natl Acad Sci U S A. 1988. PMID: 2839834 Free PMC article. - L3T4 and the immunoglobulin gene superfamily: new relationships between the immune system and the nervous system.
Parnes JR, Hunkapiller T. Parnes JR, et al. Immunol Rev. 1987 Dec;100:109-27. doi: 10.1111/j.1600-065x.1987.tb00529.x. Immunol Rev. 1987. PMID: 3326818 Review.
Cited by
- Coreceptors and TCR Signaling - the Strong and the Weak of It.
Mørch AM, Bálint Š, Santos AM, Davis SJ, Dustin ML. Mørch AM, et al. Front Cell Dev Biol. 2020 Oct 16;8:597627. doi: 10.3389/fcell.2020.597627. eCollection 2020. Front Cell Dev Biol. 2020. PMID: 33178706 Free PMC article. Review. - Evolving immune circuits are generated by flexible, motile, and sequential immunological synapses.
Gérard A, Beemiller P, Friedman RS, Jacobelli J, Krummel MF. Gérard A, et al. Immunol Rev. 2013 Jan;251(1):80-96. doi: 10.1111/imr.12021. Immunol Rev. 2013. PMID: 23278742 Free PMC article. Review. - Visualization of Cell-Cell Interaction Contacts: Synapses and Kinapses.
Dustin ML. Dustin ML. Self Nonself. 2011 Apr;2(2):85-97. doi: 10.4161/self.2.2.17931. Epub 2011 Apr 1. Self Nonself. 2011. PMID: 22299060 Free PMC article. - Co-receptors and recognition of self at the immunological synapse.
Gascoigne NR, Zal T, Yachi PP, Hoerter JA. Gascoigne NR, et al. Curr Top Microbiol Immunol. 2010;340:171-89. doi: 10.1007/978-3-642-03858-7_9. Curr Top Microbiol Immunol. 2010. PMID: 19960314 Free PMC article. Review.
References
- Proc Natl Acad Sci U S A. 1982 Jun;79(11):3604-7 - PubMed
- J Mol Cell Immunol. 1986;2(4):179-90 - PubMed
- J Cell Biol. 1983 Aug;97(2):359-67 - PubMed
- J Exp Med. 1983 Sep 1;158(3):836-56 - PubMed
- J Immunol. 1985 Jun;134(6):3994-4000 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials