Role of plasminogen, plasmin, and plasminogen activators in the migration of fibroblasts into plasma clots - PubMed (original) (raw)
Role of plasminogen, plasmin, and plasminogen activators in the migration of fibroblasts into plasma clots
P Knox et al. J Cell Physiol. 1987 Sep.
Abstract
Human diploid fibroblasts were seeded onto or into plasma clots and different aspects of cell adhesion and migration were measured. The roles of plasminogen activators and plasmin were studied by either the removal of plasminogen from plasma prior to clotting or by the addition of 10 mM epsilon-aminocaproic acid, which brings about an inhibition of plasmin in this system. When cells were seeded onto the surface of plasma clots, rates of attachment, spreading, and migration were unaffected by plasminogen depletion or plasmin inhibition. In contrast, when cells were seeded into plasma clots, then, although the rates of cells spreading were unaffected, cell migration was abolished by plasminogen depletion or by plasmin inhibition. When cells were seeded onto the surface of plasma clots and the rate of migration into the clots was measured, there was an absolute requirement for plasmin activity; while fibroblasts migrated rapidly into the fibrin lattice of control clots, in the case of plasminogen-depleted clots, cells failed to penetrate the lattice. Focussing through a plasma clot revealed that fibroblasts do not migrate through the fibrin lattice but instead, localized areas of fibrinolysis are generated and cells migrate over the surface of the area of lysis.
Similar articles
- Plasminogen activation and lysis of blood clots induced by cells in vitro.
Knox P, Crooks S. Knox P, et al. J Cell Physiol. 1988 Jun;135(3):467-73. doi: 10.1002/jcp.1041350314. J Cell Physiol. 1988. PMID: 2456288 - Incorporation of fragment X into fibrin clots renders them more susceptible to lysis by plasmin.
Schaefer AV, Leslie BA, Rischke JA, Stafford AR, Fredenburgh JC, Weitz JI. Schaefer AV, et al. Biochemistry. 2006 Apr 4;45(13):4257-65. doi: 10.1021/bi0525730. Biochemistry. 2006. PMID: 16566600 - Fibrinolysis--a review.
Kane KK. Kane KK. Ann Clin Lab Sci. 1984 Nov-Dec;14(6):443-9. Ann Clin Lab Sci. 1984. PMID: 6239587 Review. - Molecular mechanisms of fibrinolysis and their application to fibrin-specific thrombolytic therapy.
Collen D. Collen D. J Cell Biochem. 1987 Feb;33(2):77-86. doi: 10.1002/jcb.240330202. J Cell Biochem. 1987. PMID: 3553213 Review.
Cited by
- Matrix metalloproteinases (MMPs) regulate fibrin-invasive activity via MT1-MMP-dependent and -independent processes.
Hotary KB, Yana I, Sabeh F, Li XY, Holmbeck K, Birkedal-Hansen H, Allen ED, Hiraoka N, Weiss SJ. Hotary KB, et al. J Exp Med. 2002 Feb 4;195(3):295-308. doi: 10.1084/jem.20010815. J Exp Med. 2002. PMID: 11828004 Free PMC article. - Fibroblast migration in fibrin gel matrices.
Brown LF, Lanir N, McDonagh J, Tognazzi K, Dvorak AM, Dvorak HF. Brown LF, et al. Am J Pathol. 1993 Jan;142(1):273-83. Am J Pathol. 1993. PMID: 8424460 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources