A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissues - PubMed (original) (raw)
A retrovirus expressing the 12S adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissues
R D Cone et al. Mol Cell Biol. 1988 Mar.
Abstract
An epithelial cell-transforming virus could be of great use, both in the culture of epithelial cell lines and in the study of carcinogenesis. Since the adenoviral E1A gene has been shown to partially transform some epithelial cells from primary rat cell cultures, we constructed retrovirus vectors containing either the 12S or 13S E1A cDNA sequences to facilitate the transfer of these genes into a variety of primary cell types. The 12S E1A virus induced proliferation and immortalization of epithelial cells in rat kidney, liver, heart, pancreas, and thyroid primary cultures. In the two cases tested, heart and liver cultures, E1A-immortalized cells were nontumorigenic, but could be completely transformed by subsequent introduction of the ras oncogene. To our surprise, the 13S virus had a greatly reduced immortalization potential. We discuss these data in light of the model of Spindler et al. (K. R. Spindler, C. Y. Eng, and A.-J. Berk, J. Virol. 53:742-750, 1985), in which the 12S E1A protein is required for the complete induction of the cellular DNA replication machinery in the quiescent human epithelial cells in which adenoviruses normally replicate.
Similar articles
- Lytic and transforming functions of individual products of the adenovirus E1A gene.
Moran E, Grodzicker T, Roberts RJ, Mathews MB, Zerler B. Moran E, et al. J Virol. 1986 Mar;57(3):765-75. doi: 10.1128/JVI.57.3.765-775.1986. J Virol. 1986. PMID: 2936898 Free PMC article. - Growth factor induction by the adenovirus type 5 E1A 12S protein is required for immortalization of primary epithelial cells.
Quinlan MP, Whyte P, Grodzicker T. Quinlan MP, et al. Mol Cell Biol. 1988 Aug;8(8):3191-203. doi: 10.1128/mcb.8.8.3191-3203.1988. Mol Cell Biol. 1988. PMID: 2974923 Free PMC article. - Adenovirus E1A 12S protein induces DNA synthesis and proliferation in primary epithelial cells in both the presence and absence of serum.
Quinlan MP, Grodzicker T. Quinlan MP, et al. J Virol. 1987 Mar;61(3):673-82. doi: 10.1128/JVI.61.3.673-682.1987. J Virol. 1987. PMID: 3027395 Free PMC article. - Immortalization of primary baby rat kidney cells by retroviral mediated gene transfer of adenovirus E1A genes.
Lynch MJ, Trainer DL. Lynch MJ, et al. Cancer Res. 1989 Oct 1;49(19):5429-34. Cancer Res. 1989. PMID: 2766307
Cited by
- Neuroretinal Cell Culture Model as a Tool for the Development of New Therapeutic Approaches for Oxidative Stress-Induced Ocular Diseases, with a Focus on Glaucoma.
Fomo KN, Perumal N, Manicam C, Pfeiffer N, Grus FH. Fomo KN, et al. Cells. 2024 May 1;13(9):775. doi: 10.3390/cells13090775. Cells. 2024. PMID: 38727311 Free PMC article. - Cell Immortality: In Vitro Effective Techniques to Achieve and Investigate Its Applications and Challenges.
Chalak M, Hesaraki M, Mirbahari SN, Yeganeh M, Abdi S, Rajabi S, Hemmatzadeh F. Chalak M, et al. Life (Basel). 2024 Mar 21;14(3):417. doi: 10.3390/life14030417. Life (Basel). 2024. PMID: 38541741 Free PMC article. Review. - An immortalized microglial cell line (Mocha) derived from rat cochlea.
Seigel GM, Manohar S, Bai YY, Ding D, Salvi R. Seigel GM, et al. Mol Cell Neurosci. 2017 Dec;85:202-210. doi: 10.1016/j.mcn.2017.11.001. Epub 2017 Nov 3. Mol Cell Neurosci. 2017. PMID: 29109020 Free PMC article. - Review: R28 retinal precursor cells: the first 20 years.
Seigel GM. Seigel GM. Mol Vis. 2014 Mar 14;20:301-6. eCollection 2014. Mol Vis. 2014. PMID: 24644404 Free PMC article. Review. - High-throughput microtiter assay for Hoechst 33342 dye uptake.
Seigel GM, Campbell LM. Seigel GM, et al. Cytotechnology. 2004 Jul;45(3):155-60. doi: 10.1007/s10616-004-7256-9. Cytotechnology. 2004. PMID: 19003252 Free PMC article.
References
- Invest Ophthalmol. 1968 Aug;7(4):357-65 - PubMed
- J Virol. 1986 Mar;57(3):765-75 - PubMed
- Int J Cancer. 1980 May 15;25(5):631-9 - PubMed
- Nature. 1979 Oct 25;281(5733):694-6 - PubMed
- Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:383-99 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials