Chlorate: a reversible inhibitor of proteoglycan sulfation - PubMed (original) (raw)
Chlorate: a reversible inhibitor of proteoglycan sulfation
D E Humphries et al. Biochem Biophys Res Commun. 1988.
Abstract
Bovine aorta endothelial cells were cultured in medium containing [3H]glucosamine, [35S]sulfate, and various concentrations of chlorate. Cell growth was not affected by 10 mM chlorate, while 30 mM chlorate had a slight inhibitory effect. Chlorate concentrations greater than 10 mM resulted in significant undersulfation of chondroitin. With 30 mM chlorate, sulfation of chondroitin was reduced to 10% and heparan to 35% of controls, but [3H]glucosamine incorporation on a per cell basis did not appear to be inhibited. Removal of chlorate from the culture medium of cells resulted in the rapid resumption of sulfation.
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