Sertoli cell processes have axoplasmic features: an ordered microtubule distribution and an abundant high molecular weight microtubule-associated protein (cytoplasmic dynein) - PubMed (original) (raw)
Comparative Study
Sertoli cell processes have axoplasmic features: an ordered microtubule distribution and an abundant high molecular weight microtubule-associated protein (cytoplasmic dynein)
M D Neely et al. J Cell Biol. 1988 Nov.
Abstract
Microtubules in the cytoplasm of rat Sertoli cell stage VI-VIII testicular seminiferous epithelium were studied morphometrically by electron microscopy. The Sertoli cell microtubules demonstrated axonal features, being largely parallel in orientation and predominantly spaced one to two microtubule diameters apart, suggesting the presence of microtubule-bound spacer molecules. Testis microtubule-associated proteins (MAPs) were isolated by a taxol, salt elution procedure. Testis MAPs promoted microtubule assembly, but to a lesser degree than brain MAPs. High molecular weight MAPs, similar in electrophoretic mobilities to brain MAP-1 and MAP-2, were prominent components of total testis MAPs, though no shared immunoreactivity was detected between testis and brain high molecular weight MAPs using both polyclonal and monoclonal antibodies. Unlike brain high molecular weight MAPs, testis high molecular weight MAPs were not heat stable. Testis MAP composition, studied on postnatal days 5, 10, 15, and 24 and in the adult, changed dramatically during ontogeny. However, the expression of the major testis high molecular weight MAP, called HMW-2, was constitutive and independent of the development of mature germ cells. The Sertoli cell origin of HMW-2 was confirmed by identifying this protein as the major MAP found in an enriched Sertoli cell preparation and in two rat models of testicular injury characterized by germ cell depletion. HMW-2 was selectively released from testis microtubules by ATP and co-purified by sucrose density gradient centrifugation with MAP-1C, a neuronal cytoplasmic dynein. The inhibition of the microtubule-activated ATPase activity of HMW-2 by vanadate and erythro-(2-hydroxy-3-nonyl)adenine and its proteolytic breakdown by vanadate-dependent UV photocleavage confirmed the dynein-like nature of HMW-2. As demonstrated by this study, the neuronal and Sertoli cell cytoskeletons share morphological, structural and functional properties.
Similar articles
- Preparation of microtubules from rat liver and testis: cytoplasmic dynein is a major microtubule associated protein.
Collins CA, Vallee RB. Collins CA, et al. Cell Motil Cytoskeleton. 1989;14(4):491-500. doi: 10.1002/cm.970140407. Cell Motil Cytoskeleton. 1989. PMID: 2560414 - Characterization of the microtubule-activated ATPase of brain cytoplasmic dynein (MAP 1C).
Shpetner HS, Paschal BM, Vallee RB. Shpetner HS, et al. J Cell Biol. 1988 Sep;107(3):1001-9. doi: 10.1083/jcb.107.3.1001. J Cell Biol. 1988. PMID: 2971069 Free PMC article. - MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties.
Paschal BM, Shpetner HS, Vallee RB. Paschal BM, et al. J Cell Biol. 1987 Sep;105(3):1273-82. doi: 10.1083/jcb.105.3.1273. J Cell Biol. 1987. PMID: 2958482 Free PMC article. - Role of microtubule-associated proteins in the control of microtubule assembly.
Maccioni RB, Cambiazo V. Maccioni RB, et al. Physiol Rev. 1995 Oct;75(4):835-64. doi: 10.1152/physrev.1995.75.4.835. Physiol Rev. 1995. PMID: 7480164 Review. - Higher plant microtubule-associated proteins: in vitro functional assays.
Vantard M, Schellenbaum P, Peter C, Lambert AM. Vantard M, et al. Biochimie. 1993;75(8):725-30. doi: 10.1016/0300-9084(93)90103-y. Biochimie. 1993. PMID: 8286444 Review.
Cited by
- Characterization of CCDC103 expression profiles: further insights in primary ciliary dyskinesia and in human reproduction.
Pereira R, Oliveira ME, Santos R, Oliveira E, Barbosa T, Santos T, Gonçalves P, Ferraz L, Pinto S, Barros A, Oliveira J, Sousa M. Pereira R, et al. J Assist Reprod Genet. 2019 Aug;36(8):1683-1700. doi: 10.1007/s10815-019-01509-7. Epub 2019 Jun 29. J Assist Reprod Genet. 2019. PMID: 31273583 Free PMC article. - Assessment of doxorubicin-induced mouse testicular damage by the novel second-harmonic generation microscopy.
Yang CC, Chen YT, Chen CH, Chiang JY, Zhen YY, Yip HK. Yang CC, et al. Am J Transl Res. 2017 Dec 15;9(12):5275-5288. eCollection 2017. Am J Transl Res. 2017. PMID: 29312482 Free PMC article. - Testicular histopathology associated with disruption of the Sertoli cell cytoskeleton.
Johnson KJ. Johnson KJ. Spermatogenesis. 2015 Feb 19;4(2):e979106. doi: 10.4161/21565562.2014.979106. eCollection 2014 May-Aug. Spermatogenesis. 2015. PMID: 26413393 Free PMC article. Review. - MAP/microtubule affinity-regulating kinases, microtubule dynamics, and spermatogenesis.
Tang EI, Mruk DD, Cheng CY. Tang EI, et al. J Endocrinol. 2013 Apr 15;217(2):R13-23. doi: 10.1530/JOE-12-0586. Print 2013 May. J Endocrinol. 2013. PMID: 23449618 Free PMC article. Review. - KATNAL1 regulation of sertoli cell microtubule dynamics is essential for spermiogenesis and male fertility.
Smith LB, Milne L, Nelson N, Eddie S, Brown P, Atanassova N, O'Bryan MK, O'Donnell L, Rhodes D, Wells S, Napper D, Nolan P, Lalanne Z, Cheeseman M, Peters J. Smith LB, et al. PLoS Genet. 2012;8(5):e1002697. doi: 10.1371/journal.pgen.1002697. Epub 2012 May 24. PLoS Genet. 2012. PMID: 22654668 Free PMC article.
References
- Nature. 1970 Aug 15;227(5259):680-5 - PubMed
- J Cell Biol. 1984 Dec;99(6):2287-96 - PubMed
- Proc Natl Acad Sci U S A. 1975 Jan;72(1):177-81 - PubMed
- Proc Natl Acad Sci U S A. 1975 Jul;72(7):2696-700 - PubMed
- Biochemistry. 1976 Oct 5;15(20):4497-505 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials