Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells - PubMed (original) (raw)

Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells

E J Bowman et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

Various membrane ATPases have been tested for their sensitivity to bafilomycin A1, a macrolide antibiotic. F1F0 ATPases from bacteria and mitochondria are not affected by this antibiotic. In contrast, E1E2 ATPases--e.g., the K+-dependent (Kdp) ATPase from Escherichia coli, the Na+,K+-ATPase from ox brain, and the Ca2+-ATPase from sarcoplasmic reticulum--are moderately sensitive to this inhibitor. Finally, membrane ATPases from Neurospora vacuoles, chromaffin granules, and plant vacuoles are extremely sensitive. From this we conclude that bafilomycin A1 is a valuable tool for distinguishing among the three different types of ATPases and represents the first relatively specific potent inhibitor of vacuolar ATPases.

PubMed Disclaimer

References

    1. Anal Biochem. 1976 Mar;71(1):209-13 - PubMed
    1. Anal Biochem. 1975 Mar;64(1):136-41 - PubMed
    1. J Biol Chem. 1985 Jul 25;260(15):8726-30 - PubMed
    1. Biochem J. 1985 Nov 1;231(3):557-64 - PubMed
    1. Plant Physiol. 1985 Jun;78(2):327-33 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources