Human papillomavirus type 16 recombinant DNA is maintained as an autonomously replicating episome in monkey kidney cells - PubMed (original) (raw)

Human papillomavirus type 16 recombinant DNA is maintained as an autonomously replicating episome in monkey kidney cells

P M Chesters et al. J Gen Virol. 1985 Mar.

Abstract

Human papillomavirus type 16 (HPV16) DNA cloned in the expression vector pSV2-neo has been shown to be maintained in transfected monkey kidney cells as an autonomously replicating episome at a level of 2 to 10 copies per cell. Integration of pSV2-neo/HPV16 DNA with the host genome occurred in transfected mouse fibroblasts. Neither type of cell appeared to be phenotypically transformed.

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