Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors - PubMed (original) (raw)
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
C Yanisch-Perron et al. Gene. 1985.
Erratum in
- Corrected nucleotide sequence of M13mp18 gene III.
Ebright R, Dong Q, Messing J. Ebright R, et al. Gene. 1992 May 1;114(1):81-3. doi: 10.1016/0378-1119(92)90710-7. Gene. 1992. PMID: 1375182
Abstract
Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences. A new suppressorless strain facilitates the cloning of selected recombinants. (2) The complete nucleotide sequences of the M13mp and pUC vectors have been compiled from a number of sources, including the sequencing of selected segments. The M13mp18 sequence is revised to include the G-to-T substitution in its gene II at position 6 125 bp (in M13) or 6967 bp in M13mp18. (3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII.
Similar articles
- Cloning in M13 phage or how to use biology at its best.
Messing J. Messing J. Gene. 1991 Apr;100:3-12. doi: 10.1016/0378-1119(91)90344-b. Gene. 1991. PMID: 2055478 Review. - M13 vectors for selective cloning of sequences specifying initiation of DNA synthesis on single-stranded templates.
Ray DS, Hines JC, Kim MH, Imber R, Nomura N. Ray DS, et al. Gene. 1982 Jun;18(3):231-8. doi: 10.1016/0378-1119(82)90160-3. Gene. 1982. PMID: 6290326 - Jekyll, a family of phage-plasmid shuttle vectors.
Burmeister M, Lehrach H. Burmeister M, et al. Gene. 1988 Dec 15;73(1):245-50. doi: 10.1016/0378-1119(88)90331-9. Gene. 1988. PMID: 2854093 - Plasmid pKUN9, a versatile vector for the selective packaging of both DNA strands into single-stranded DNA-containing phage-like particles.
Peeters BP, Schoenmakers JG, Konings RN. Peeters BP, et al. Gene. 1986;41(1):39-46. doi: 10.1016/0378-1119(86)90265-9. Gene. 1986. PMID: 3009274 - [A simple system of cloning in phage M13 and DNA sequencing with terminators].
Kraev AS. Kraev AS. Mol Biol (Mosk). 1988 Sep-Oct;22(5):1164-97. Mol Biol (Mosk). 1988. PMID: 3065614 Review. Russian.
Cited by
- MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems.
Sladitschek HL, Neveu PA. Sladitschek HL, et al. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PLoS One. 2015. PMID: 25909630 Free PMC article. - Sul1 and Sul2 sulfate transceptors signal to protein kinase A upon exit of sulfur starvation.
Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM. Kankipati HN, et al. J Biol Chem. 2015 Apr 17;290(16):10430-46. doi: 10.1074/jbc.M114.629022. Epub 2015 Feb 27. J Biol Chem. 2015. PMID: 25724649 Free PMC article. - A Conserved Class II Type Thioester Domain-Containing Adhesin Is Required for Efficient Conjugation in Bacillus subtilis.
Gago-Córdoba C, Val-Calvo J, Abia D, Díaz-Talavera A, Miguel-Arribas A, Aguilar Suárez R, van Dijl JM, Wu LJ, Meijer WJJ. Gago-Córdoba C, et al. mBio. 2021 Mar 16;12(2):e00104-21. doi: 10.1128/mBio.00104-21. mBio. 2021. PMID: 33727345 Free PMC article. - Genome sequencing-assisted identification and the first functional validation of N-acyl-homoserine-lactone synthases from the Sphingomonadaceae family.
Gan HM, Dailey LK, Halliday N, Williams P, Hudson AO, Savka MA. Gan HM, et al. PeerJ. 2016 Aug 30;4:e2332. doi: 10.7717/peerj.2332. eCollection 2016. PeerJ. 2016. PMID: 27635318 Free PMC article. - Identification of a novel gene product that promotes survival of Mycobacterium smegmatis in macrophages.
Pelosi A, Smith D, Brammananth R, Topolska A, Billman-Jacobe H, Nagley P, Crellin PK, Coppel RL. Pelosi A, et al. PLoS One. 2012;7(2):e31788. doi: 10.1371/journal.pone.0031788. Epub 2012 Feb 21. PLoS One. 2012. PMID: 22363734 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials