Topoisomerase II is a structural component of mitotic chromosome scaffolds - PubMed (original) (raw)

Topoisomerase II is a structural component of mitotic chromosome scaffolds

W C Earnshaw et al. J Cell Biol. 1985 May.

Abstract

We have obtained a polyclonal antibody that recognizes a major polypeptide component of chicken mitotic chromosome scaffolds. This polypeptide migrates in SDS PAGE with Mr 170,000. Indirect immunofluorescence and subcellular fractionation experiments confirm that it is present in both mitotic chromosomes and interphase nuclei. Two lines of evidence suggest that this protein is DNA topoisomerase II, an abundant nuclear enzyme that controls DNA topological states: anti-scaffold antibody inhibits the strand-passing activity of DNA topoisomerase II; and both anti-scaffold antibody and an independent antibody raised against purified bovine topoisomerase II recognize identical partial proteolysis fragments of the 170,000-mol-wt scaffold protein in immunoblots. Our results suggest that topoisomerase II may be an enzyme that is also a structural protein of interphase nuclei and mitotic chromosomes.

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References

1. 1. Chromosoma. 1975 Nov 11;52(4):329-38 - PubMed
2. 1. Methods Cell Biol. 1975;12:335-51 - PubMed
3. 1. Cell. 1976 Nov;9(3):393-407 - PubMed
4. 1. Cell. 1977 Nov;12(3):805-16 - PubMed
5. 1. Cell. 1977 Nov;12(3):817-28 - PubMed

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