A whole-blood lymphoproliferation assay for measuring cellular immunity against herpes viruses - PubMed (original) (raw)
A whole-blood lymphoproliferation assay for measuring cellular immunity against herpes viruses
M Leroux et al. J Immunol Methods. 1985.
Abstract
A whole blood test system was established to study cell-mediated immunity to cytomegalovirus (CMV) and herpes simplex virus (HSV) in a large number of healthy blood donors. Cellular immunity was measured by the in vitro proliferative response (LP) of peripheral lymphocytes. These responded vigorously to several mitogens. Lymphocytes of most individuals responded to HSV, but only a limited number were reactive towards CMV. In parallel, antibodies against CMV and HSV were measured by an ELISA technique. For HSV, good correlation was observed between serological and lymphocyte proliferation results. For CMV, no clear correlation was obtained, only 21 of 40 donors positive in the antibody test being positive in the LP test. The majority of seronegatives were negative in the LP test. Use of virions purified by sucrose gradient centrifugation, or an additional strain of CMV (strain Davis) did not increase the number of donors positive in the LP test. One explanation might be that individuals possessing antibodies against CMV as measured by ELISA but no capacity to react in the LP test had suffered from a CMV infection a long time before, and now showed waning cellular immunity, but antibody still detectable. Use of the whole blood technique on 108 individuals showed that this very simple test works well with various mitogens and at least some antigens.
Similar articles
- In vitro stimulation of human lymphocytes by purified cytomegalovirus.
Moller-Larsen A, Andersen HK, Heron I, Sarov I. Moller-Larsen A, et al. Intervirology. 1975-1976;6(4-5):249-57. doi: 10.1159/000149479. Intervirology. 1975. PMID: 186434 - Cellular immune response to human cytomegalovirus. I. Lymphocyte transformation studies in the rabbit.
Lausch RN, Jones C, Christensen RC. Lausch RN, et al. Intervirology. 1979;11(2):74-81. doi: 10.1159/000149016. Intervirology. 1979. PMID: 85612 - Studies of cellular immunity, serum interferon titers, and natural killer cell activity in schizophrenic patients.
Schindler L, Leroux M, Beck J, Moises HW, Kirchner H. Schindler L, et al. Acta Psychiatr Scand. 1986 Jun;73(6):651-7. doi: 10.1111/j.1600-0447.1986.tb02739.x. Acta Psychiatr Scand. 1986. PMID: 2428210 - Lymphocyte proliferation and IgG production with herpesvirus antigens in solid phase.
Ljungman P, Wahren B, Sundqvist VA. Ljungman P, et al. J Virol Methods. 1985 Dec;12(3-4):199-208. doi: 10.1016/0166-0934(85)90130-2. J Virol Methods. 1985. PMID: 2422193
Cited by
- Cellular immunity to nucleoproteins (NP) of Crimean-Congo hemorrhagic fever virus (CCHFV) and Hazara Virus (HAZV).
Kalkan-Yazıcı M, Karaaslan E, Güler-Çetin NS, Doymaz MZ. Kalkan-Yazıcı M, et al. Med Microbiol Immunol. 2024 Sep 25;213(1):20. doi: 10.1007/s00430-024-00802-2. Med Microbiol Immunol. 2024. PMID: 39320473 - A standardized method to study immune responses using porcine whole blood.
Mattoo SU, Aganja RP, Kim SC, Jeong CG, Nazki S, Khatun A, Kim WI, Lee SM. Mattoo SU, et al. J Vet Sci. 2023 Jan;24(1):e11. doi: 10.4142/jvs.22210. J Vet Sci. 2023. PMID: 36726276 Free PMC article. - Plasma glutamate levels, lymphocyte reactivity and death rate in patients with bronchial carcinoma.
Eck HP, Drings P, Dröge W. Eck HP, et al. J Cancer Res Clin Oncol. 1989;115(6):571-4. doi: 10.1007/BF00391360. J Cancer Res Clin Oncol. 1989. PMID: 2558118 - T-cell proliferative response to human papillomavirus type 16 peptides: relationship to cervical intraepithelial neoplasia.
Nakagawa M, Stites DP, Farhat S, Judd A, Moscicki AB, Canchola AJ, Hilton JF, Palefsky JM. Nakagawa M, et al. Clin Diagn Lab Immunol. 1996 Mar;3(2):205-10. doi: 10.1128/cdli.3.2.205-210.1996. Clin Diagn Lab Immunol. 1996. PMID: 8991637 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources