Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family - PubMed (original) (raw)
Comparative Study
Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family
P Fort et al. Nucleic Acids Res. 1985.
Free PMC article
Abstract
We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5' and 3' non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.
Similar articles
- Sequence analysis of the cloned mRNA coding for glyceraldehyde-3-phosphate dehydrogenase from chicken heart muscle.
Domdey H, Wiebauer K, Klapthor H, Arnold HH. Domdey H, et al. Eur J Biochem. 1983 Mar 1;131(1):129-35. doi: 10.1111/j.1432-1033.1983.tb07239.x. Eur J Biochem. 1983. PMID: 6832136 - Glyceraldehyde 3-phosphate dehydrogenase protein and mRNA are both differentially expressed in adult chickens but not chick embryos.
Milner RJ, Brow MD, Cleveland DW, Shinnick TM, Sutcliffe JG. Milner RJ, et al. Nucleic Acids Res. 1983 May 25;11(10):3301-15. doi: 10.1093/nar/11.10.3301. Nucleic Acids Res. 1983. PMID: 6687938 Free PMC article. - Cloning and sequencing of a deoxyribonucleic acid copy of glyceraldehyde-3-phosphate dehydrogenase messenger ribonucleic acid isolated from chicken muscle.
Dugaiczyk A, Haron JA, Stone EM, Dennison OE, Rothblum KN, Schwartz RJ. Dugaiczyk A, et al. Biochemistry. 1983 Mar 29;22(7):1605-13. doi: 10.1021/bi00276a013. Biochemistry. 1983. PMID: 6303388 - Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene.
Tso JY, Sun XH, Kao TH, Reece KS, Wu R. Tso JY, et al. Nucleic Acids Res. 1985 Apr 11;13(7):2485-502. doi: 10.1093/nar/13.7.2485. Nucleic Acids Res. 1985. PMID: 2987855 Free PMC article. - Control selection for RNA quantitation.
Suzuki T, Higgins PJ, Crawford DR. Suzuki T, et al. Biotechniques. 2000 Aug;29(2):332-7. doi: 10.2144/00292rv02. Biotechniques. 2000. PMID: 10948434 Review.
Cited by
- Bioenergetic Failure in Rat Oligodendrocyte Progenitor Cells Treated with Cerebrospinal Fluid Derived from Multiple Sclerosis Patients.
Mathur D, Riffo-Campos AL, Castillo J, Haines JD, Vidaurre OG, Zhang F, Coret-Ferrer F, Casaccia P, Casanova B, Lopez-Rodas G. Mathur D, et al. Front Cell Neurosci. 2017 Jul 18;11:209. doi: 10.3389/fncel.2017.00209. eCollection 2017. Front Cell Neurosci. 2017. PMID: 28775680 Free PMC article. - Selection and Validation of Reference Genes for Quantitative Real-time PCR in Gentiana macrophylla.
He Y, Yan H, Hua W, Huang Y, Wang Z. He Y, et al. Front Plant Sci. 2016 Jun 29;7:945. doi: 10.3389/fpls.2016.00945. eCollection 2016. Front Plant Sci. 2016. PMID: 27446172 Free PMC article. - Bypassing hazard of housekeeping genes: their evaluation in rat granule neurons treated with cerebrospinal fluid of multiple sclerosis subjects.
Mathur D, Urena-Peralta JR, Lopez-Rodas G, Casanova B, Coret-Ferrer F, Burgal-Marti M. Mathur D, et al. Front Cell Neurosci. 2015 Sep 23;9:375. doi: 10.3389/fncel.2015.00375. eCollection 2015. Front Cell Neurosci. 2015. PMID: 26441545 Free PMC article. - Validation of Tuba1a as appropriate internal control for normalization of gene expression analysis during mouse lung development.
Mehta A, Dobersch S, Dammann RH, Bellusci S, Ilinskaya ON, Braun T, Barreto G. Mehta A, et al. Int J Mol Sci. 2015 Feb 25;16(3):4492-511. doi: 10.3390/ijms16034492. Int J Mol Sci. 2015. PMID: 25723738 Free PMC article.
References
- Anal Biochem. 1976 May 7;72:413-27 - PubMed
- Nucleic Acids Res. 1984 Dec 11;12(23):9179-89 - PubMed
- Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
- Annu Rev Biochem. 1977;46:573-639 - PubMed
- Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials