Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene - PubMed (original) (raw)

Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene

J Y Tso et al. Nucleic Acids Res. 1985.

Free PMC article

Abstract

Full length cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rat and man have been isolated and sequenced. Many GAPDH gene-related sequences have been found in both genomes based on genomic blot hybridization analysis. Only one functional gene product is known. Results from genomic library screenings suggest that there are 300-400 copies of these sequences in the rat genome and approximately 100 in the human genome. Some of these related sequences have been shown to be processed pseudogenes. We have isolated several rat cDNA clones corresponding to these pseudogenes indicating that some pseudogenes are transcribed. Rat and human cDNAs are 89% homologous in the coding region, and 76% homologous in the first 100 base pairs of the 3'-noncoding region. Comparison of these two cDNA sequences with those of the chicken, Drosophila and yeast genes allows the analysis of the evolution of the GAPDH genes in detail.

PubMed Disclaimer

Similar articles

• Unusual abundance of vertebrate 3-phosphate dehydrogenase pseudogenes.
  Piechaczyk M, Blanchard JM, Riaad-El Sabouty S, Dani C, Marty L, Jeanteur P. Piechaczyk M, et al. Nature. 1984 Nov 29-Dec 5;312(5993):469-71. doi: 10.1038/312469a0. Nature. 1984. PMID: 6095107
• Structure of two unlinked Drosophila melanogaster glyceraldehyde-3-phosphate dehydrogenase genes.
  Tso JY, Sun XH, Wu R. Tso JY, et al. J Biol Chem. 1985 Jul 5;260(13):8220-8. J Biol Chem. 1985. PMID: 2989282
• Transcriptional mapping of two yeast genes coding for glyceraldehyde 3-phosphate dehydrogenase isolated by sequence homology with the chicken gene.
  Musti AM, Zehner Z, Bostian KA, Paterson BM, Kramer RA. Musti AM, et al. Gene. 1983 Nov;25(1):133-43. doi: 10.1016/0378-1119(83)90175-0. Gene. 1983. PMID: 6363210
• Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene.
  Ercolani L, Florence B, Denaro M, Alexander M. Ercolani L, et al. J Biol Chem. 1988 Oct 25;263(30):15335-41. J Biol Chem. 1988. PMID: 3170585
• Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.
  Fort P, Marty L, Piechaczyk M, el Sabrouty S, Dani C, Jeanteur P, Blanchard JM. Fort P, et al. Nucleic Acids Res. 1985 Mar 11;13(5):1431-42. doi: 10.1093/nar/13.5.1431. Nucleic Acids Res. 1985. PMID: 2987824 Free PMC article.

Cited by

• Notch suppresses nuclear factor of activated T cells (NFAT) transactivation and Nfatc1 expression in chondrocytes.
  Zanotti S, Canalis E. Zanotti S, et al. Endocrinology. 2013 Feb;154(2):762-72. doi: 10.1210/en.2012-1925. Epub 2012 Dec 21. Endocrinology. 2013. PMID: 23264614 Free PMC article.
• Dietary level of protein regulates glyceraldehyde-3-phosphate dehydrogenase content and synthesis rate in mouse liver cytosol.
  Sanllorenti PM, Tardivo DB, Conde RD. Sanllorenti PM, et al. Mol Cell Biochem. 1992 Oct 7;115(2):117-28. doi: 10.1007/BF00230321. Mol Cell Biochem. 1992. PMID: 1448056
• Structure and expression of human mitochondrial adenylate kinase targeted to the mitochondrial matrix.
  Noma T, Fujisawa K, Yamashiro Y, Shinohara M, Nakazawa A, Gondo T, Ishihara T, Yoshinobu K. Noma T, et al. Biochem J. 2001 Aug 15;358(Pt 1):225-32. doi: 10.1042/0264-6021:3580225. Biochem J. 2001. PMID: 11485571 Free PMC article.
• Functional mechanism underlying COX-2 expression following administration of indomethacin in rat stomachs: importance of gastric hypermotility.
  Takeuchi K, Tanaka A, Hayashi Y, Kubo Y. Takeuchi K, et al. Dig Dis Sci. 2004 Feb;49(2):180-7. doi: 10.1023/b:ddas.0000017436.05273.fd. Dig Dis Sci. 2004. PMID: 15104355

References

1. 1. J Mol Biol. 1982 Jul 15;158(4):573-97 - PubMed
2. 1. J Biol Chem. 1982 Mar 25;257(6):3026-31 - PubMed
3. 1. Cell. 1983 Feb;32(2):473-82 - PubMed
4. 1. J Biol Chem. 1983 Apr 25;258(8):5291-9 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1983 Apr;80(7):1845-8 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource

• Research Materials

  • NCI CPTC Antibody Characterization Program