Primary isolation media for Legionnaires disease bacterium - PubMed (original) (raw)

Primary isolation media for Legionnaires disease bacterium

J C Feeley et al. J Clin Microbiol. 1978 Sep.

Abstract

Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% Iso Vitale X and 1% hemoglobin (MH-IH). This medium was subsequently analyzed to determine the components required to support growth of the LDB. L-Cysteine hydrochloride can replace the Iso Vitale X reagent, and soluble ferric pyrophosphate can replace hemoglobin. A new medium, F-G agar, was formulated incorporating these chemicals. Different cultures conditions (oxygen tension, temperature, and pH) were also evaluated. The LDB grew optimally at 35 degrees C under 2.5% CO2 on the F-G agar adjusted to pH 6.9. When infected tissues were inoculated onto both F-G agar and MH-IH, the F-G agar produced colonies of the LDB more rapidly and in greater numbers than did MH-IH.

PubMed Disclaimer

Similar articles

• Virulent to avirulent conversion of Legionnaires' disease bacterium (Legionella pneumophila)--its effect on isolation techniques.
  McDade JE, Shepard CC. McDade JE, et al. J Infect Dis. 1979 Jun;139(6):707-11. doi: 10.1093/infdis/139.6.707. J Infect Dis. 1979. PMID: 448196
• Legionnaires' disease bacterium isolated in 1947.
  McDade JE, Brenner DJ, Bozeman FM. McDade JE, et al. Ann Intern Med. 1979 Apr;90(4):659-61. doi: 10.7326/0003-4819-90-4-659. Ann Intern Med. 1979. PMID: 373548
• Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila.
  Feeley JC, Gibson RJ, Gorman GW, Langford NC, Rasheed JK, Mackel DC, Baine WB. Feeley JC, et al. J Clin Microbiol. 1979 Oct;10(4):437-41. doi: 10.1128/jcm.10.4.437-441.1979. J Clin Microbiol. 1979. PMID: 393713 Free PMC article.
• [Bacteriology of Legionnaires' disease].
  Stefanini A, Galassi L. Stefanini A, et al. Recenti Prog Med. 1981 May;70(5):557-8. Recenti Prog Med. 1981. PMID: 7031802 Review. Italian. No abstract available.
• [Legionnaires' disease, a previously unknown infectious disease of bacterial nature].
  Prozorovskii SV. Prozorovskii SV. Zh Mikrobiol Epidemiol Immunobiol. 1979 Feb;(2):8-14. Zh Mikrobiol Epidemiol Immunobiol. 1979. PMID: 369246 Review. Russian. No abstract available.

Cited by

• Improvements to the Success of Outbreak Investigations of Legionnaires' Disease: 40 Years of Testing and Investigation in New York State.
  Schoonmaker-Bopp D, Nazarian E, Dziewulski D, Clement E, Baker DJ, Dickinson MC, Saylors A, Codru N, Thompson L, Lapierre P, Dumas N, Limberger R, Musser KA. Schoonmaker-Bopp D, et al. Appl Environ Microbiol. 2021 Jul 27;87(16):e0058021. doi: 10.1128/AEM.00580-21. Epub 2021 Jul 27. Appl Environ Microbiol. 2021. PMID: 34085864 Free PMC article.
• Comparison of BCYEα+AB agar and MWY agar for detection and enumeration of Legionella spp. in hospital water samples.
  Ditommaso S, Giacomuzzi M, Memoli G, Garlasco J, Zotti CM. Ditommaso S, et al. BMC Microbiol. 2021 Feb 16;21(1):48. doi: 10.1186/s12866-021-02109-1. BMC Microbiol. 2021. PMID: 33593295 Free PMC article.
• Functional characterization of the HMP-P synthase of Legionella pneumophila (Lpg1565).
  Paxhia MD, Swanson MS, Downs DM. Paxhia MD, et al. Mol Microbiol. 2021 Apr;115(4):539-553. doi: 10.1111/mmi.14622. Epub 2020 Nov 3. Mol Microbiol. 2021. PMID: 33034117 Free PMC article.
• Chlorine Disinfection of Legionella spp., L. pneumophila, and Acanthamoeba under Warm Water Premise Plumbing Conditions.
  Martin RL, Harrison K, Proctor CR, Martin A, Williams K, Pruden A, Edwards MA. Martin RL, et al. Microorganisms. 2020 Sep 22;8(9):1452. doi: 10.3390/microorganisms8091452. Microorganisms. 2020. PMID: 32971988 Free PMC article.
• Temperature-Dependent Growth Modeling of Environmental and Clinical Legionella pneumophila Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Genotypes.
  Sharaby Y, Rodríguez-Martínez S, Oks O, Pecellin M, Mizrahi H, Peretz A, Brettar I, Höfle MG, Halpern M. Sharaby Y, et al. Appl Environ Microbiol. 2017 Mar 31;83(8):e03295-16. doi: 10.1128/AEM.03295-16. Print 2017 Apr 15. Appl Environ Microbiol. 2017. PMID: 28159784 Free PMC article.

References

1. 1. Adv Protein Chem. 1956;11:311-427 - PubMed
2. 1. N Engl J Med. 1977 Dec 1;297(22):1189-97 - PubMed
3. 1. N Engl J Med. 1977 Dec 1;297(22):1197-203 - PubMed
4. 1. N Engl J Med. 1977 Dec 1;297(22):1218-20 - PubMed
5. 1. J Clin Microbiol. 1977 Aug;6(2):140-3 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database