Epstein-Barr virus infections and DNA hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: the role of primary infection - PubMed (original) (raw)

Epstein-Barr virus infections and DNA hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: the role of primary infection

M Ho et al. J Infect Dis. 1985 Nov.

Abstract

Fourteen patients who developed B cell lymphomas or lymphoproliferative lesions after kidney, liver, heart, or heart-lung transplantation in Pittsburgh during 1981-1983 had active infection with Epstein-Barr virus (EBV) of the primary (six patients), reactivated (seven patients), or chronic (one patient) type. In transplant patients without tumors, the incidence of EBV infection was 30% (39 of 128). Only three of these patients had primary infections. Thus the frequency of active infection was significantly higher in patients with tumors, and patients with primary infections were at greater risk of developing tumors. Five of 13 tumors tested contained EBV nuclear antigen (EBNA) and nine of 11 contained EBV genomes detected by DNA-DNA hybridization with BamHI K, BamHI W, or EcoRI B cloned probes. All EBNA-positive tumors, except one, were also positive by hybridization. Only one tumor was negative for both EBNA and EBV DNA. These data suggest that EBV plays an etiologic role in the development of these lesions.

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Figures

Figure 1

Southern blot analysis of tumor tissue from organ transplant recipients with the EBV probe _Eco_RI B. Lane A contains the equivalent of 330 copies per cell of probe DNA in a sample of 106 cells. Lane D contains DNA from a lymphoid line containing 40 genome copies per cell; the DNA from 105 cells was loaded in this lane. Lane E contains DNA from an EBV-negative cell line. Lanes F and G contain 1 µg and 500 ng, respectively, of DNA from the lung biopsy of a heart transplant recipient (patient 13); lanes H and I contain 1 µg and 500 ng, respectively, of DNA from a lymph node of a heart-lung transplant recipient (patient 6); lanes J and K contain 1 µg and 500 ng of DNA from one tonsil, and lanes L and M contain 1 µg and 500 ng of DNA from the other tonsil of patient 5, a heart-lung transplant recipient. EBV DNA was found in tissues from patients 13 and 5 but not 6. Lanes G, K, and M each contain the DNA equivalents of 105 cells. The signals corresponding to these three samples represent the equivalent of ~40 copies per cell. Note that all samples contain a group of _Bam_HI fragments expected to be present in _Eco_RI B probe.

Figure 2

Southern blot analysis with the EBV probe _Bam_HI K. Titration of the probe is found in lanes a–c. Lane a contains 100 pg of the probe. Lane b contains 10 pg of _Bam_HI K, the equivalent of one genome copy per cell in 106 cells. Lane c contains 1 pg. Lane d contains DNA from a lymphoid line containing 40 copies per cell; the DNA from 105 cells was loaded in this lane. Thus, the signal is equivalent to four copies per cell in a sample of 106 cells. Lane e is a negative control. Lanes f–i contain 5 µg of DNA from each of four patients (7, 11, 9, 8); this is ~ 106 cell equivalents of DNA per lane. Thus, samples f, g, and i have 10 copies per cell, and sample h has four copies per cell. Lanes j and k contain DNA from an unrelated patient. The _Bam_HI K fragment is known to vary in size.

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Epstein-Barr virus infections and DNA hybridization studies in posttransplantation lymphoma and lymphoproliferative lesions: the role of primary infection - PubMed (original) (raw)