Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure - PubMed (original) (raw)
Chromatin reconstituted from tandemly repeated cloned DNA fragments and core histones: a model system for study of higher order structure
R T Simpson et al. Cell. 1985 Oct.
Abstract
We describe a model system for study of chromatin structure at levels above that of the nucleosome. A series of fragments with lengths ranging from 172 to 207 bp tandemly repeated three to greater than 50 times was prepared; each repeat contains the region important in forming a positioned core particle on a sea urchin 5S rRNA gene upon in vitro association with histones. The tandemly repeated sequences can be studied as linear DNA fragments or as relaxed or supercoiled circular molecules. A number of criteria indicate that nucleosomes position correctly on all the tandemly repeated elements. Measurement of the change in linking number per core particle led to a value of -1.0. Both length and repeat number dependent changes in conformation of the nucleoproteins are observed. We discuss the possibility that some ordered higher level chromatin structure can form with DNA and core histones alone.
Similar articles
- Conformation of nucleosome core particles and chromatin in high salt concentration.
Wilhelm ML, Wilhelm FX. Wilhelm ML, et al. Biochemistry. 1980 Sep 2;19(18):4327-31. doi: 10.1021/bi00559a028. Biochemistry. 1980. PMID: 7417408 - Chromatosome positioning on assembled long chromatin. Linker histones affect nucleosome placement on 5 S rDNA.
Meersseman G, Pennings S, Bradbury EM. Meersseman G, et al. J Mol Biol. 1991 Jul 5;220(1):89-100. doi: 10.1016/0022-2836(91)90383-h. J Mol Biol. 1991. PMID: 2067021 - DNA sequence organization in chromatosomes.
Muyldermans S, Travers AA. Muyldermans S, et al. J Mol Biol. 1994 Jan 21;235(3):855-70. doi: 10.1006/jmbi.1994.1044. J Mol Biol. 1994. PMID: 8289324 - Chromatin reconstitution on small DNA rings. II. DNA supercoiling on the nucleosome.
Zivanovic Y, Goulet I, Revet B, Le Bret M, Prunell A. Zivanovic Y, et al. J Mol Biol. 1988 Mar 20;200(2):267-90. doi: 10.1016/0022-2836(88)90239-2. J Mol Biol. 1988. PMID: 2836595 - Histone-DNA interactions in erythrocyte chromatin.
Reeck GR. Reeck GR. Arch Biochem Biophys. 1976 Jan;172(1):117-22. doi: 10.1016/0003-9861(76)90055-2. Arch Biochem Biophys. 1976. PMID: 1252069 No abstract available.
Cited by
- In vitro co-expression chromatin assembly and remodeling platform for plant histone variants.
Banko P, Okimune KI, Nagy SK, Hamasaki A, Morishita R, Onouchi H, Takasuka TE. Banko P, et al. Sci Rep. 2024 Jan 10;14(1):936. doi: 10.1038/s41598-024-51460-6. Sci Rep. 2024. PMID: 38195981 Free PMC article. - DNA-binding proteins studied by mechanical manipulation and AFM imaging of single DNA molecules.
Zhao X, Liu XP, Yan J. Zhao X, et al. Biophys Rep. 2022 Aug 31;8(4):212-224. doi: 10.52601/bpr.2022.220015. Biophys Rep. 2022. PMID: 37288005 Free PMC article. - Hydrozoan sperm-specific SPKK motif-containing histone H2B variants stabilise chromatin with limited compaction.
Török A, Browne MJG, Vilar JC, Patwal I, DuBuc TQ, Febrimarsa, Atcheson E, Frank U, Gornik SG, Flaus A. Török A, et al. Development. 2023 Jan 1;150(1):dev201058. doi: 10.1242/dev.201058. Epub 2023 Jan 12. Development. 2023. PMID: 36633190 Free PMC article. - Histone variants and chromatin structure, update of advances.
Sokolova V, Sarkar S, Tan D. Sokolova V, et al. Comput Struct Biotechnol J. 2022 Dec 5;21:299-311. doi: 10.1016/j.csbj.2022.12.002. eCollection 2023. Comput Struct Biotechnol J. 2022. PMID: 36582440 Free PMC article. Review. - The solid and liquid states of chromatin.
Hansen JC, Maeshima K, Hendzel MJ. Hansen JC, et al. Epigenetics Chromatin. 2021 Oct 30;14(1):50. doi: 10.1186/s13072-021-00424-5. Epigenetics Chromatin. 2021. PMID: 34717733 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous