Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection - PubMed (original) (raw)
Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection
R D Schrier et al. Science. 1985.
Abstract
In situ hybridization was used to detect human cytomegalovirus (HCMV) in the peripheral blood mononuclear cells of some naturally infected (seropositive) individuals. A subpopulation of cells hybridized specifically to a portion of the HCMV genome that is heavily transcribed during the immediate-early period of infection. The hybridization signal was markedly reduced by base hydrolysis and ribonuclease, and therefore the probe appears to be detecting viral RNA. A fluorescence-activated cell sorter was used to select lymphocytes bearing the OKT4 and OKT8 markers. Hybridization with the HCMV probe revealed a higher proportion of positive cells in the OKT4 than in the OKT8 subset. This observation specifically identifies lymphocytes as a cell population involved in natural HCMV infection and suggests that lymphocytes may be a reservoir for maintaining infection and may also serve as a vehicle for its spread by blood transfusion.
Similar articles
- Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization.
Dankner WM, McCutchan JA, Richman DD, Hirata K, Spector SA. Dankner WM, et al. J Infect Dis. 1990 Jan;161(1):31-6. doi: 10.1093/infdis/161.1.31. J Infect Dis. 1990. PMID: 2153186 - Definition of a subset of human peripheral blood mononuclear cells that are permissive to human cytomegalovirus infection.
Söderberg C, Larsson S, Bergstedt-Lindqvist S, Möller E. Söderberg C, et al. J Virol. 1993 Jun;67(6):3166-75. doi: 10.1128/JVI.67.6.3166-3175.1993. J Virol. 1993. PMID: 7684461 Free PMC article. - Chronically persistent infection with human cytomegalovirus in human lymphoblasts.
Furukawa T, Yoshimura N, Jean JH, Plotkin SA. Furukawa T, et al. J Infect Dis. 1979 Feb;139(2):211-4. doi: 10.1093/infdis/139.2.211. J Infect Dis. 1979. PMID: 220333 - Detection of human cytomegalovirus in urine by DNA-DNA and RNA-DNA hybridization.
Schuster V, Matz B, Wiegand H, Traub B, Kampa D, Neumann-Haefelin D. Schuster V, et al. J Infect Dis. 1986 Aug;154(2):309-14. doi: 10.1093/infdis/154.2.309. J Infect Dis. 1986. PMID: 3014011 - Detection of human cytomegalovirus DNA: how, when and where?
Gerna G, Baldanti F, Zella D, Furione M. Gerna G, et al. Scand J Infect Dis Suppl. 1995;99:11-5. Scand J Infect Dis Suppl. 1995. PMID: 8668932 Review.
Cited by
- Human Hematopoietic Long-Term Culture (hLTC) for Human Cytomegalovirus Latency and Reactivation.
Peppenelli M, Buehler J, Goodrum F. Peppenelli M, et al. Methods Mol Biol. 2021;2244:83-101. doi: 10.1007/978-1-0716-1111-1_5. Methods Mol Biol. 2021. PMID: 33555583 Free PMC article. - A Novel Human Skin Tissue Model To Study Varicella-Zoster Virus and Human Cytomegalovirus.
Lloyd MG, Smith NA, Tighe M, Travis KL, Liu D, Upadhyaya PK, Kinchington PR, Chan GC, Moffat JF. Lloyd MG, et al. J Virol. 2020 Oct 27;94(22):e01082-20. doi: 10.1128/JVI.01082-20. Print 2020 Oct 27. J Virol. 2020. PMID: 32878893 Free PMC article. - A molecular mechanism for probabilistic bet hedging and its role in viral latency.
Chaturvedi S, Klein J, Vardi N, Bolovan-Fritts C, Wolf M, Du K, Mlera L, Calvert M, Moorman NJ, Goodrum F, Huang B, Weinberger LS. Chaturvedi S, et al. Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17240-17248. doi: 10.1073/pnas.1914430117. Epub 2020 Jul 6. Proc Natl Acad Sci U S A. 2020. PMID: 32632017 Free PMC article. - A novel murine model of differentiation-mediated cytomegalovirus reactivation from latently infected bone marrow haematopoietic cells.
Liu XF, Swaminathan S, Yan S, Engelmann F, Abbott DA, VanOsdol LA, Heald-Sargent T, Qiu L, Chen Q, Iovane A, Zhang Z, Abecassis MM. Liu XF, et al. J Gen Virol. 2019 Dec;100(12):1680-1694. doi: 10.1099/jgv.0.001327. J Gen Virol. 2019. PMID: 31647403 Free PMC article. - HCMV modulation of cellular PI3K/AKT/mTOR signaling: New opportunities for therapeutic intervention?
Altman AM, Mahmud J, Nikolovska-Coleska Z, Chan G. Altman AM, et al. Antiviral Res. 2019 Mar;163:82-90. doi: 10.1016/j.antiviral.2019.01.009. Epub 2019 Jan 19. Antiviral Res. 2019. PMID: 30668978 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical