Cellular DNA regions involved in the induction of rat thymic lymphomas (Mlvi-1, Mlvi-2, Mlvi-3, and c-myc) represent independent loci as determined by their chromosomal map location in the rat - PubMed (original) (raw)

Cellular DNA regions involved in the induction of rat thymic lymphomas (Mlvi-1, Mlvi-2, Mlvi-3, and c-myc) represent independent loci as determined by their chromosomal map location in the rat

P N Tsichlis et al. J Virol. 1985 Dec.

Abstract

The induction of thymic lymphomas by Moloney murine leukemia virus in the rat is linked to provirus integration in at least four independent cellular DNA regions (Mlvi-1, Mlvi-2, Mlvi-3, and c-myc). Because sequences homologous to at least three of these regions (Mlvi-1, Mlvi-2, and c-myc) map to chromosome 15 in the mouse, the question was raised whether they are closely linked in the rat genome and whether provirus integration in any one of these regions affects the same functional domain in rat DNA. In this study, we identified the chromosomal map location of Mlvi-1, Mlvi-2, and Mlvi-3 in the rat by using mouse-rat somatic cell hybrids that lose the rat chromosomes. The results showed that Mlvi-1 maps similarly to c-myc to chromosome 7, and Mlvi-2 maps to chromosome 2. Mlvi-3 probably maps to chromosome 15. We conclude that Mlvi-1, Mlvi-2, and Mlvi-3 are separate and independent genetic loci. Although Mlvi-1 and c-myc map to the same chromosome, they are not related, as determined by hybridization and restriction endonuclease mapping. The chromosomal map location of Mlvi-1 to chromosome 7 and Mlvi-2 to chromosome 2 is interesting, since chromosomal aberrations involving these two chromosomes are reproducibly observed in rat neoplasias induced by a variety of agents.

PubMed Disclaimer

Cited by

Correlation between genetic and cytogenetic maps of the rat.
Andoh Y, Kuramoto T, Yokoi N, Maihara T, Kitada K, Serikawa T. Andoh Y, et al. Mamm Genome. 1998 Apr;9(4):287-93. doi: 10.1007/s003359900750. Mamm Genome. 1998. PMID: 9530624
Localization of a Moloney murine leukemia virus integration site gene, Mlvi2, on rat chromosome 2.
Kunieda T, Kobayashi E, Tachibana M, Ikadai H. Kunieda T, et al. Mamm Genome. 1996 Dec;7(12):924-5. doi: 10.1007/s003359900276. Mamm Genome. 1996. PMID: 8995772 No abstract available.
A rat genetic linkage map and comparative maps for mouse or human homologous rat genes.
Yamada J, Kuramoto T, Serikawa T. Yamada J, et al. Mamm Genome. 1994 Feb;5(2):63-83. doi: 10.1007/BF00292332. Mamm Genome. 1994. PMID: 8180477 Free PMC article. No abstract available.
Dsi-1, a region with frequent proviral insertions in Moloney murine leukemia virus-induced rat thymomas.
Vijaya S, Steffen DL, Kozak C, Robinson HL. Vijaya S, et al. J Virol. 1987 Apr;61(4):1164-70. doi: 10.1128/JVI.61.4.1164-1170.1987. J Virol. 1987. PMID: 3029411 Free PMC article.

References

1. Hereditas. 1976;84(1):1-14 - PubMed
1. J Natl Cancer Inst. 1978 Jun;60(6):1399-404 - PubMed
1. J Virol. 1980 Jan;33(1):238-49 - PubMed
1. Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:1123-32 - PubMed
1. Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:1133-41 - PubMed

Cellular DNA regions involved in the induction of rat thymic lymphomas (Mlvi-1, Mlvi-2, Mlvi-3, and c-myc) represent independent loci as determined by their chromosomal map location in the rat - PubMed (original) (raw)