Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia - PubMed (original) (raw)
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia
R K Saiki et al. Science. 1985.
Abstract
Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies. In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end-labeled oligonucleotide probe hybridized in solution to the amplified beta-globin sequences. The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.
Similar articles
- Rapid prenatal diagnosis of sickle cell anemia by a new method of DNA analysis.
Embury SH, Scharf SJ, Saiki RK, Gholson MA, Golbus M, Arnheim N, Erlich HA. Embury SH, et al. N Engl J Med. 1987 Mar 12;316(11):656-61. doi: 10.1056/NEJM198703123161103. N Engl J Med. 1987. PMID: 3821796 - Diagnosis of sickle cell anemia and beta-thalassemia with enzymatically amplified DNA and nonradioactive allele-specific oligonucleotide probes.
Saiki RK, Chang CA, Levenson CH, Warren TC, Boehm CD, Kazazian HH Jr, Erlich HA. Saiki RK, et al. N Engl J Med. 1988 Sep 1;319(9):537-41. doi: 10.1056/NEJM198809013190903. N Engl J Med. 1988. PMID: 3405266 - Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. 1985.
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Saiki RK, et al. Biotechnology. 1992;24:476-80. Biotechnology. 1992. PMID: 1422056 No abstract available. - Prenatal diagnosis of the common haemoglobin disorders.
Weatherall DJ, Old JM, Thein SL, Wainscoat JS, Clegg JB. Weatherall DJ, et al. J Med Genet. 1985 Dec;22(6):422-30. doi: 10.1136/jmg.22.6.422. J Med Genet. 1985. PMID: 3001310 Free PMC article. Review. - Prenatal diagnosis of thalassemias and hemoglobinopathies.
Seale TW, Rennert OM. Seale TW, et al. Ann Clin Lab Sci. 1980 Sep-Oct;10(5):383-94. Ann Clin Lab Sci. 1980. PMID: 6252819 Review.
Cited by
- Sweet enhancers of polymerase chain reaction.
Xie B, Chen J, Wang Z, Yin Q, Dai ZM. Xie B, et al. PLoS One. 2024 Oct 29;19(10):e0311939. doi: 10.1371/journal.pone.0311939. eCollection 2024. PLoS One. 2024. PMID: 39471151 Free PMC article. - Investigating the role of keratin proteins and microbial associations in hereditary and pathogenic alopecia.
Liquat N, Hassan MU, Shafique F, Khan S, Alanzi AR, Khan NU. Liquat N, et al. Arch Dermatol Res. 2024 Oct 26;316(10):718. doi: 10.1007/s00403-024-03436-9. Arch Dermatol Res. 2024. PMID: 39460809 - Enhancing Colorimetric Detection of Nucleic Acids on Nitrocellulose Membranes: Cutting-Edge Applications in Diagnostics and Forensics.
Subhashini N, Kerler Y, Menger MM, Böhm O, Witte J, Stadler C, Griberman A. Subhashini N, et al. Biosensors (Basel). 2024 Sep 5;14(9):430. doi: 10.3390/bios14090430. Biosensors (Basel). 2024. PMID: 39329805 Free PMC article. - Nanotechnology-Enabled PCR with Tunable Energy Dynamics.
Zhao X, Peng H, Hu J, Wang L, Zhang F. Zhao X, et al. JACS Au. 2024 Aug 30;4(9):3370-3382. doi: 10.1021/jacsau.4c00570. eCollection 2024 Sep 23. JACS Au. 2024. PMID: 39328766 Free PMC article. Review. - Evolving strategies in microbe identification-a comprehensive review of biochemical, MALDI-TOF MS and molecular testing methods.
Arbefeville SS, Timbrook TT, Garner CD. Arbefeville SS, et al. J Antimicrob Chemother. 2024 Sep 19;79(Supplement_1):i2-i8. doi: 10.1093/jac/dkae275. J Antimicrob Chemother. 2024. PMID: 39298363 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials