An Epstein-Barr virus transcription unit is at least 84 kilobases long - PubMed (original) (raw)
An Epstein-Barr virus transcription unit is at least 84 kilobases long
M Bodescot et al. Nucleic Acids Res. 1986.
Free PMC article
Abstract
We have studied the structure of the Epstein-Barr virus mRNAs expressed in B95-8, a productively-infected Marmoset cell line established from in vitro-infected B-lymphocytes. We constructed a cDNA library from the cytoplasmic polyadenylated RNAs of B95-8 in the lambda gt10 bacteriophage. We present here the analysis of a 3.5 kbp cDNA containing exons transcribed from the US, IR and UL regions of the viral genome. The corresponding transcription unit is at least 84 kbp long. Two exons are transcribed from the US region, five from the IR region and two from the UL region. The exons from the IR region consist of two tandem repeats of a unit containing two exons, 66 and 132 nucleotides, and of a third copy of the 66 nucleotide exon. The exons from the UL region contain an open reading frame coding for a 944 amino acid polypeptide. The C-terminal end of this polypeptide harbors three types of repeated sequences. The corresponding mRNA is the second described of a family of mRNAs produced by alternative splicing of exons transcribed from the US, IR and UL regions.
Similar articles
- Epstein-Barr virus mRNAs produced by alternative splicing.
Bodescot M, Perricaudet M. Bodescot M, et al. Nucleic Acids Res. 1986 Sep 11;14(17):7103-14. doi: 10.1093/nar/14.17.7103. Nucleic Acids Res. 1986. PMID: 3020506 Free PMC article. - Spliced RNA from the IR1-U2 region of Epstein-Barr virus: presence of an open reading frame for a repetitive polypeptide.
Bodescot M, Chambraud B, Farrell P, Perricaudet M. Bodescot M, et al. EMBO J. 1984 Aug;3(8):1913-7. doi: 10.1002/j.1460-2075.1984.tb02067.x. EMBO J. 1984. PMID: 6090131 Free PMC article. - Phosphonoacetic acid-resistant RNA of Epstein-Barr virus in productively infected cells.
Shin S, Donovan J, Nonoyama M. Shin S, et al. Virology. 1983 Jan 15;124(1):196-200. doi: 10.1016/0042-6822(83)90306-9. Virology. 1983. PMID: 6130649 - An Epstein-Barr virus transcript from a latently infected, growth-transformed B-cell line encodes a highly repetitive polypeptide.
Speck SH, Pfitzner A, Strominger JL. Speck SH, et al. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9298-302. doi: 10.1073/pnas.83.24.9298. Proc Natl Acad Sci U S A. 1986. PMID: 3025831 Free PMC article. - Mapping of putative transforming sequences of EBV DNA.
Kieff E, Raab-Traub N, Given D, King W, Powell AT, Pritchett R, Dambaugh T. Kieff E, et al. IARC Sci Publ (1971). 1978;(24 Pt 1):527-52. IARC Sci Publ (1971). 1978. PMID: 221358 Review.
Cited by
- Epstein-Barr virus nuclear antigen 2 specifically induces expression of the B-cell activation antigen CD23.
Wang F, Gregory CD, Rowe M, Rickinson AB, Wang D, Birkenbach M, Kikutani H, Kishimoto T, Kieff E. Wang F, et al. Proc Natl Acad Sci U S A. 1987 May;84(10):3452-6. doi: 10.1073/pnas.84.10.3452. Proc Natl Acad Sci U S A. 1987. PMID: 3033649 Free PMC article. - Definition of the sequence requirements for binding of the EBNA-1 protein to its palindromic target sites in Epstein-Barr virus DNA.
Ambinder RF, Shah WA, Rawlins DR, Hayward GS, Hayward SD. Ambinder RF, et al. J Virol. 1990 May;64(5):2369-79. doi: 10.1128/JVI.64.5.2369-2379.1990. J Virol. 1990. PMID: 2157891 Free PMC article. - Bidirectional transcription of the Epstein-Barr virus major internal repeat.
Rogers RP, Speck SH. Rogers RP, et al. J Virol. 1990 May;64(5):2426-9. doi: 10.1128/JVI.64.5.2426-2429.1990. J Virol. 1990. PMID: 1691315 Free PMC article. - Epstein-Barr virus mRNAs produced by alternative splicing.
Bodescot M, Perricaudet M. Bodescot M, et al. Nucleic Acids Res. 1986 Sep 11;14(17):7103-14. doi: 10.1093/nar/14.17.7103. Nucleic Acids Res. 1986. PMID: 3020506 Free PMC article. - The Epstein-Barr virus nuclear protein encoded by the leader of the EBNA RNAs is important in B-lymphocyte transformation.
Mannick JB, Cohen JI, Birkenbach M, Marchini A, Kieff E. Mannick JB, et al. J Virol. 1991 Dec;65(12):6826-37. doi: 10.1128/JVI.65.12.6826-6837.1991. J Virol. 1991. PMID: 1658376 Free PMC article.
References
- Nucleic Acids Res. 1980 Nov 11;8(21):4867-88 - PubMed
- Proc Natl Acad Sci U S A. 1980 May;77(5):2999-3003 - PubMed
- Annu Rev Biochem. 1981;50:349-83 - PubMed
- Annu Rev Biochem. 1981;50:555-83 - PubMed
- Nucleic Acids Res. 1982 Jan 22;10(2):459-72 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources