Growth factors activate the bumetanide-sensitive Na+/K+/Cl-cotransport in hamster fibroblasts - PubMed (original) (raw)
. 1986 May 15;261(14):6177-83.
- PMID: 3009447
Free article
Growth factors activate the bumetanide-sensitive Na+/K+/Cl-cotransport in hamster fibroblasts
S Paris et al. J Biol Chem. 1986.
Free article
Abstract
alpha-Thrombin, a potent mitogen for the hamster fibroblast cell line CCL 39, stimulates by approximately 3-fold 86Rb+ uptake in a mutant lacking the Na+/H+ antiport activity (PS 120). The major component of this stimulated 86Rb+ (K+) uptake is a bumetanide-sensitive flux (IC50 = 0.4 microM), which accounts for 50% of total K+ uptake in Go-arrested cells and is approximately 4-fold stimulated by maximal thrombin concentrations (EC50 = 5 X 10(-4) units/ml). This bumetanide-sensitive 86Rb+ uptake represents a Na+/K+/Cl- cotransport, as indicated by its dependence on extracellular Na+ and Cl- and by the existence in PS 120 cells of a bumetanide-sensitive K+-dependent 22Na+ uptake. The stimulation reaches its maximum within 2 min, is reduced at acidic intracellular pH values (half-maximal at pHi = 6.8), and can also be induced, to a lesser extent, by EGF and the phorbol ester 12-O-tetradecanoylphorbol 13-acetate, the effects of which are nearly additive. In contrast, preincubation with 12-O-tetradecanoylphorbol 13-acetate results in inhibition of thrombin- and EGF-induced stimulations, suggesting that activated protein kinase C might exert a feedback inhibitory control. This study clearly demonstrates that the growth factor-induced activation of the Na+/K+/Cl- cotransport is separated from the activation of the Na+/H+ antiport. However, activation of this cotransporter does not seem to play a major role in the mitogenic signaling pathway since its complete inhibition with bumetanide reduces only by 25-30% reinitiation of DNA synthesis.
Similar articles
- Na+/K+/Cl- cotransport is stimulated by a Ca(++)-calmodulin-mediated pathway in BALB/c 3T3 fibroblasts.
Snyder D, Atlan H, Markus M, Panet R. Snyder D, et al. J Cell Physiol. 1991 Dec;149(3):497-502. doi: 10.1002/jcp.1041490320. J Cell Physiol. 1991. PMID: 1744176 - Dopamine D1 stimulation of Na+,K+,Cl- cotransport in human NPE cells: effects of multiple hormones.
Riese K, Beyer AT, Lui GM, Crook RB. Riese K, et al. Invest Ophthalmol Vis Sci. 1998 Jul;39(8):1444-52. Invest Ophthalmol Vis Sci. 1998. PMID: 9660493 - Phorbol ester TPA inhibits the stimulation of bumetanide-sensitive Na+/K+/Cl- transporter by different mitogens in quiescent BALB/c 3T3 mouse fibroblasts.
Snyder D, Markus M, Atlan H, Panet R. Snyder D, et al. J Cell Physiol. 1991 Mar;146(3):466-72. doi: 10.1002/jcp.1041460318. J Cell Physiol. 1991. PMID: 2022701 - The mechanism of insulin stimulation of (Na+,K+)-ATPase transport activity in muscle.
Rosić NK, Standaert ML, Pollet RJ. Rosić NK, et al. J Biol Chem. 1985 May 25;260(10):6206-12. J Biol Chem. 1985. PMID: 2987218 Review.
Cited by
- Advances in the development of novel compounds targeting cation-chloride cotransporter physiology.
Delpire E. Delpire E. Am J Physiol Cell Physiol. 2021 Mar 1;320(3):C324-C340. doi: 10.1152/ajpcell.00566.2020. Epub 2020 Dec 23. Am J Physiol Cell Physiol. 2021. PMID: 33356948 Free PMC article. Review. - Emerging roles for sodium dependent amino acid transport in mesenchymal cells.
Dall'asta V, Franchi-Gazzola R, Bussolati O, Sala R, Rotoli BM, Rossi PA, Uggeri J, Belletti S, Visigalli R, Gazzola GC. Dall'asta V, et al. Amino Acids. 1996 Jun;11(2):117-33. doi: 10.1007/BF00813856. Amino Acids. 1996. PMID: 24178683 - Ca2+ signaling, intracellular pH and cell volume in cell proliferation.
Schreiber R. Schreiber R. J Membr Biol. 2005 Jun;205(3):129-37. doi: 10.1007/s00232-005-0778-z. J Membr Biol. 2005. PMID: 16362501 Review. - Na(+)-K(+)-Cl- cotransport system in brain capillary endothelial cells: response to endothelin and hypoxia.
Kawai N, McCarron RM, Spatz M. Kawai N, et al. Neurochem Res. 1996 Oct;21(10):1259-66. doi: 10.1007/BF02532403. Neurochem Res. 1996. PMID: 8923488 - Cellular mechanisms in activation of Na-K-Cl cotransport in nasal gland acinar cells of guinea pigs.
Ikeda K, Wu D, Takasaka T. Ikeda K, et al. J Membr Biol. 1995 Aug;146(3):307-14. doi: 10.1007/BF00233950. J Membr Biol. 1995. PMID: 8568845
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical