A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c - PubMed (original) (raw)
A mutation allowing an mRNA secondary structure diminishes translation of Saccharomyces cerevisiae iso-1-cytochrome c
S B Baim et al. Mol Cell Biol. 1985 Aug.
Abstract
The CYC1-239-O mutation in the yeast Saccharomyces cerevisiae produces a -His-Leu- replacement of the normal -Ala-Gly- sequence at amino acid positions 5 and 6, which lie within a dispensable region of iso-1-cytochrome c; this mutation can accommodate the formation of a hairpin structure at the corresponding site in the mRNA. The amount of the altered protein was diminished to 20% of the wild-type level, whereas the amount of the mRNA remained normal. However, in contrast to the normal CYC1+ mRNA that is associated mainly with four to seven ribosomes, the bulk of the CYC1-239-O mRNA is associated with one to four ribosomes. These results suggest that the stable secondary structure within the translated region of the CYC1 mRNA diminishes translation by inhibiting elongation.
References
- J Mol Biol. 1973 Jan;73(1):1-16 - PubMed
- Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 - PubMed
- Genetics. 1974 Jun;77(2):255-84 - PubMed
- J Biol Chem. 1974 Jul 10;249(13):4006-15 - PubMed
- Genetics. 1975 Dec;81(4):615-29 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous