Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif - PubMed (original) (raw)

Nonhomologous recombination in the parvovirus chromosome: role for a CTATTTCT motif

A Hogan et al. Mol Cell Biol. 1986 Aug.

Abstract

The mechanism of nonhomologous recombination in murine cells infected with the parvovirus minute virus of mice (MVM) has been investigated by analysis of DNA sequences at recombination junctions in naturally occurring deletion variants of the virus. We report here that nonhomologous recombination in the MVM chromosome is characterized by short homologies, by insertion at recombination junctions of foreign DNA sequences that are enriched for preferred eucaryotic topoisomerase I cleavage sites, and by an association with a common DNA sequence motif of the type 5'-CTATTTCT-3'. Additional analyses of broken MVM chromosomes provided evidence for specific enzymatic cleavage within 5'-CTTATC-3' and 5'-CTATTC-3' sequences. The results indicate that the 5'-CTATTTCT-3' motif is an important genetic element for nonhomologous recombination in the parvovirus chromosome.

PubMed Disclaimer

References

    1. Science. 1985 Nov 22;230(4728):954-8 - PubMed
    1. Cell. 1980 Oct;21(3):653-68 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:751-62 - PubMed
    1. Nucleic Acids Res. 1983 Feb 25;11(4):999-1018 - PubMed
    1. J Biol Chem. 1985 Oct 5;260(22):12320-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources