The cleavage of single-stranded DNA by the isoschizomeric restriction endonuclease HhaI and CfoI - PubMed (original) (raw)
The cleavage of single-stranded DNA by the isoschizomeric restriction endonuclease HhaI and CfoI
B Reckmann et al. Biochim Biophys Acta. 1987.
Abstract
The cleavage of single-stranded (ss) M13mp8(+) DNA by the isoschizomeric restriction endonucleases HhaI and CfoI has been investigated. The two enzymes differ considerably in their ability to cleave ssDNA. HhaI cleaves ssDNA about two orders of magnitude faster than does CfoI, although both enzymes show the same activity when assayed on double-stranded DNA. From the cleavage of oligonucleotides and of M13mp8(+) DNA fragments it is concluded that cleavage of ssDNA occurs via transiently formed double-stranded hairpin structures. A rough correlation exists between the stability of the secondary structures and the cleavage efficiency.
Similar articles
- Type II restriction endonucleases cleave single-stranded DNAs in general.
Nishigaki K, Kaneko Y, Wakuda H, Husimi Y, Tanaka T. Nishigaki K, et al. Nucleic Acids Res. 1985 Aug 26;13(16):5747-60. doi: 10.1093/nar/13.16.5747. Nucleic Acids Res. 1985. PMID: 2994012 Free PMC article. - Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-strand cleavages.
Yolov AA, Gromova ES, Kubareva EA, Potapov VK, Shabarova ZA. Yolov AA, et al. Nucleic Acids Res. 1985 Dec 20;13(24):8969-81. doi: 10.1093/nar/13.24.8969. Nucleic Acids Res. 1985. PMID: 3001655 Free PMC article. - Site-specific cleavage of single-stranded DNA by a Hemophilus restriction endonuclease.
Horiuchi K, Zinder ND. Horiuchi K, et al. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2555-8. doi: 10.1073/pnas.72.7.2555. Proc Natl Acad Sci U S A. 1975. PMID: 1058473 Free PMC article. - DNA-protein interactions: the use of synthetic oligo- and polynucleotides for studying single-stranded-DNA-binding proteins and restriction endonucleases.
Gromova ES, Shabarova ZA. Gromova ES, et al. Prog Nucleic Acid Res Mol Biol. 1990;39:1-47. doi: 10.1016/s0079-6603(08)60622-4. Prog Nucleic Acid Res Mol Biol. 1990. PMID: 2174177 Review. No abstract available. - Type II restriction endonucleases.
Perona JJ. Perona JJ. Methods. 2002 Nov;28(3):353-64. doi: 10.1016/s1046-2023(02)00242-6. Methods. 2002. PMID: 12431439 Review.
Cited by
- Targeted DNA methylation of neurodegenerative disease genes via homology directed repair.
Cali CP, Park DS, Lee EB. Cali CP, et al. Nucleic Acids Res. 2019 Dec 16;47(22):11609-11622. doi: 10.1093/nar/gkz979. Nucleic Acids Res. 2019. PMID: 31680172 Free PMC article. - Binary control of enzymatic cleavage of DNA origami by structural antideterminants.
Stopar A, Coral L, Di Giacomo S, Adedeji AF, Castronovo M. Stopar A, et al. Nucleic Acids Res. 2018 Jan 25;46(2):995-1006. doi: 10.1093/nar/gkx1204. Nucleic Acids Res. 2018. PMID: 29216375 Free PMC article. - Perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA) acutely affect human α1β2γ2L GABAA receptor and spontaneous neuronal network function in vitro.
Tukker AM, Bouwman LMS, van Kleef RGDM, Hendriks HS, Legler J, Westerink RHS. Tukker AM, et al. Sci Rep. 2020 Mar 24;10(1):5311. doi: 10.1038/s41598-020-62152-2. Sci Rep. 2020. PMID: 32210279 Free PMC article. - Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.
Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. Elhai J, et al. J Bacteriol. 1997 Mar;179(6):1998-2005. doi: 10.1128/jb.179.6.1998-2005.1997. J Bacteriol. 1997. PMID: 9068647 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases