From Golden Rice to aSTARice: Bioengineering Astaxanthin Biosynthesis in Rice Endosperm - PubMed (original) (raw)
. 2018 Dec 3;11(12):1440-1448.
doi: 10.1016/j.molp.2018.09.007. Epub 2018 Oct 6.
Affiliations
- PMID: 30296601
- DOI: 10.1016/j.molp.2018.09.007
Free article
From Golden Rice to aSTARice: Bioengineering Astaxanthin Biosynthesis in Rice Endosperm
Qinlong Zhu et al. Mol Plant. 2018.
Free article
Abstract
Carotenoids are important phytonutrients with antioxidant properties, and are widely used in foods and feedstuffs as supplements. Astaxanthin, a red-colored ketocarotenoid, has strong antioxidant activity and thus can benefit human health. However, astaxanthin is not produced in most higher plants. Here we report the bioengineering of astaxanthin biosynthesis in rice endosperm by introducing four synthetic genes, sZmPSY1, sPaCrtI, sCrBKT, and sHpBHY, which encode the enzymes phytoene synthase, phytoene desaturase, β-carotene ketolase, and β-carotene hydroxylase, respectively. Transgneic overexpression of two (sZmPSY1 and sPaCrtI), three (sZmPSY1, sPaCrtI and sCrBKT), and all these four genes driven by rice endosperm-specific promoters established the carotenoid/ketocarotenoid/astaxanthin biosynthetic pathways in the endosperm and thus resulted in various types of germplasm, from the yellow-grained β-carotene-enriched Golden Rice to orange-red-grained Canthaxanthin Rice and Astaxanthin Rice, respectively. Grains of Astaxanthin Rice were enriched with astaxanthin in the endosperm and had higher antioxidant activity. These results proved that introduction of a minimal set of four transgenes enables de novo biosynthesis of astaxanthin in the rice endosperm. This work provides a successful example for synthetic biology in plants and biofortification in crops; the biofortified rice products generated by this study could be consumed as health-promoting foods and processed to produce dietary supplements.
Keywords: astaxanthin biosynthesis; biofortification; multigene metabolic engineering; rice endosperm.
Copyright © 2018 The Author. Published by Elsevier Inc. All rights reserved.
Similar articles
- Reconstruction of the astaxanthin biosynthesis pathway in rice endosperm reveals a metabolic bottleneck at the level of endogenous β-carotene hydroxylase activity.
Bai C, Berman J, Farre G, Capell T, Sandmann G, Christou P, Zhu C. Bai C, et al. Transgenic Res. 2017 Feb;26(1):13-23. doi: 10.1007/s11248-016-9977-x. Epub 2016 Aug 27. Transgenic Res. 2017. PMID: 27567632 - Stepwise pathway engineering to the biosynthesis of zeaxanthin, astaxanthin and capsanthin in rice endosperm.
Ha SH, Kim JK, Jeong YS, You MK, Lim SH, Kim JK. Ha SH, et al. Metab Eng. 2019 Mar;52:178-189. doi: 10.1016/j.ymben.2018.11.012. Epub 2018 Nov 29. Metab Eng. 2019. PMID: 30503392 - Rice callus as a high-throughput platform for synthetic biology and metabolic engineering of carotenoids.
Zhu C, Bai C, Gomez-Gomez L, Sandmann G, Baysal C, Capell T, Christou P. Zhu C, et al. Methods Enzymol. 2022;671:511-526. doi: 10.1016/bs.mie.2021.09.016. Epub 2021 Oct 25. Methods Enzymol. 2022. PMID: 35878992 - Golden Rice: introducing the beta-carotene biosynthesis pathway into rice endosperm by genetic engineering to defeat vitamin A deficiency.
Beyer P, Al-Babili S, Ye X, Lucca P, Schaub P, Welsch R, Potrykus I. Beyer P, et al. J Nutr. 2002 Mar;132(3):506S-510S. doi: 10.1093/jn/132.3.506S. J Nutr. 2002. PMID: 11880581 Review. - A multifocal approach towards understanding the complexities of carotenoid biosynthesis and accumulation in rice grains.
Chettry U, Chrungoo NK. Chettry U, et al. Brief Funct Genomics. 2020 Jul 29;19(4):324-335. doi: 10.1093/bfgp/elaa007. Brief Funct Genomics. 2020. PMID: 32240289 Review.
Cited by
- Heterologous Expression of Sunflower HaHPT and HaTMT Genes Enhances Rice-Grain Vitamin E Content.
Song S, Li H, Lin S, Dong X, Tian R, Wu Z, Li Q, Li M, Zhang K, Liu X, Wan J, Liu L. Song S, et al. Plants (Basel). 2024 Aug 27;13(17):2392. doi: 10.3390/plants13172392. Plants (Basel). 2024. PMID: 39273875 Free PMC article. - Exploring the benefits of astaxanthin as a functional food ingredient: Its effects on oxidative stress and reproductive outcomes in women with PCOS - A systematic review and single-arm meta-analysis of randomized clinical trials.
Rodrigues VD, Boaro BL, Laurindo LF, Chagas EFB, de Lima EP, Laurindo LF, Barbalho SM. Rodrigues VD, et al. Naunyn Schmiedebergs Arch Pharmacol. 2024 Sep 13. doi: 10.1007/s00210-024-03432-w. Online ahead of print. Naunyn Schmiedebergs Arch Pharmacol. 2024. PMID: 39269488 Review. - Improvement and application of vacuum-infiltration system in tomato.
Yao X, Taheri A, Liu H, Zhang Y, Li L, Shao J, Wu K, Miao Q, He W, Hu X, Tang K. Yao X, et al. Hortic Res. 2024 Jul 26;11(9):uhae197. doi: 10.1093/hr/uhae197. eCollection 2024 Sep. Hortic Res. 2024. PMID: 39257545 Free PMC article. - Preferentially expressed endosperm genes reveal unique activities in wheat endosperm during grain filling.
Shi J, Zhao Y, Zhao P, Yang H, Wang C, Xia J, Zhao Z, Wang Z, Yang Z, Wang Z, Xu S, Zhang Y. Shi J, et al. BMC Genomics. 2024 Aug 22;25(1):795. doi: 10.1186/s12864-024-10713-4. BMC Genomics. 2024. PMID: 39174916 Free PMC article. - Improving Rice Grain Quality Through Ecotype Breeding for Enhancing Food and Nutritional Security in Asia-Pacific Region.
Alam M, Lou G, Abbas W, Osti R, Ahmad A, Bista S, Ahiakpa JK, He Y. Alam M, et al. Rice (N Y). 2024 Aug 5;17(1):47. doi: 10.1186/s12284-024-00725-9. Rice (N Y). 2024. PMID: 39102064 Free PMC article. Review.