Nucleotide sequence of the Escherichia coli mutH gene - PubMed (original) (raw)

Nucleotide sequence of the Escherichia coli mutH gene

R H Grafstrom et al. Nucleic Acids Res. 1987.

Abstract

The complete nucleotide sequence of mutH gene from E. coli has been determined. Based on the deduced amino acid sequence, the MutH protein has a molecular weight of 25.4 kdaltons in agreement with the previous estimates based on SDS-polyacrylamide gel electrophoresis of the purified protein. Deletion analysis of the DNA sequences upstream of mutH has identified the promoter region for this gene. Two independently isolated temperature sensitive alleles of the mutH gene have also been sequenced. One mutation results in an amino acid change at position 27 (thr to leu) while the other occurs at position 156 (asp to asn).

PubMed Disclaimer

Similar articles

• The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli.
  Ishii S, Ihara M, Maekawa T, Nakamura Y, Uchida H, Imamoto F. Ishii S, et al. Nucleic Acids Res. 1984 Apr 11;12(7):3333-42. doi: 10.1093/nar/12.7.3333. Nucleic Acids Res. 1984. PMID: 6326058 Free PMC article.
• Haemophilus influenzae and Vibrio cholerae genes for mutH are able to fully complement a mutH defect in Escherichia coli.
  Friedhoff P, Sheybani B, Thomas E, Merz C, Pingoud A. Friedhoff P, et al. FEMS Microbiol Lett. 2002 Feb 19;208(1):123-8. doi: 10.1111/j.1574-6968.2002.tb11071.x. FEMS Microbiol Lett. 2002. PMID: 11934505
• Nucleotide sequence of the xanthine guanine phosphoribosyl transferase gene of E. coli.
  Richardson KK, Fostel J, Skopek TR. Richardson KK, et al. Nucleic Acids Res. 1983 Dec 20;11(24):8809-16. doi: 10.1093/nar/11.24.8809. Nucleic Acids Res. 1983. PMID: 6324102 Free PMC article.
• Nucleotide sequence of the Escherichia coli replication gene dnaZX.
  Yin KC, Blinkowa A, Walker JR. Yin KC, et al. Nucleic Acids Res. 1986 Aug 26;14(16):6541-9. doi: 10.1093/nar/14.16.6541. Nucleic Acids Res. 1986. PMID: 3018672 Free PMC article.
• Nucleotide sequence and LexA regulation of the Escherichia coli recN gene.
  Rostas K, Morton SJ, Picksley SM, Lloyd RG. Rostas K, et al. Nucleic Acids Res. 1987 Jul 10;15(13):5041-9. doi: 10.1093/nar/15.13.5041. Nucleic Acids Res. 1987. PMID: 3037486 Free PMC article.

Cited by

• Linkage map of Escherichia coli K-12, edition 8.
  Bachmann BJ. Bachmann BJ. Microbiol Rev. 1990 Jun;54(2):130-97. doi: 10.1128/mr.54.2.130-197.1990. Microbiol Rev. 1990. PMID: 2194094 Free PMC article. Review.
• Systematic characterization of curved DNA segments randomly cloned from Escherichia coli and their functional significance.
  Tanaka K, Muramatsu S, Yamada H, Mizuno T. Tanaka K, et al. Mol Gen Genet. 1991 May;226(3):367-76. doi: 10.1007/BF00260648. Mol Gen Genet. 1991. PMID: 1903834
• Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases.
  Ban C, Yang W. Ban C, et al. EMBO J. 1998 Mar 2;17(5):1526-34. doi: 10.1093/emboj/17.5.1526. EMBO J. 1998. PMID: 9482749 Free PMC article.
• Molecular characterization of the Lactococcus lactis LlaKR2I restriction-modification system and effect of an IS982 element positioned between the restriction and modification genes.
  Twomey DP, McKay LL, O'Sullivan DJ. Twomey DP, et al. J Bacteriol. 1998 Nov;180(22):5844-54. doi: 10.1128/JB.180.22.5844-5854.1998. J Bacteriol. 1998. PMID: 9811640 Free PMC article.
• Mutants with temperature-sensitive defects in the Escherichia coli mismatch repair system: sensitivity to mispairs generated in vivo.
  Hong ES, Yeung A, Funchain P, Slupska MM, Miller JH. Hong ES, et al. J Bacteriol. 2005 Feb;187(3):840-6. doi: 10.1128/JB.187.3.840-846.2005. J Bacteriol. 2005. PMID: 15659661 Free PMC article.

References

1. 1. J Bacteriol. 1985 Sep;163(3):1007-15 - PubMed
2. 1. Annu Rev Genet. 1979;13:319-53 - PubMed
3. 1. Gene. 1984 Feb;27(2):183-91 - PubMed
4. 1. Gene. 1983 May-Jun;22(2-3):245-53 - PubMed
5. 1. J Mol Biol. 1977 Jul;114(1):153-68 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Medical

  • MedlinePlus Health Information

• Molecular Biology Databases

  • BioCyc