Construction of a human ventricular cDNA library and characterization of a beta myosin heavy chain cDNA clone - PubMed (original) (raw)

• PMID: 3032769
• DOI: 10.1007/BF00283049

Construction of a human ventricular cDNA library and characterization of a beta myosin heavy chain cDNA clone

M A Jandreski et al. Hum Genet. 1987 May.

Abstract

We have constructed and characterized for the first time a complementary DNA (cDNA) clone, pHMC3, which codes for a cardiac myosin heavy chain mRNA from human heart. This clone contains a 1.7 kb DNA segment and specifies 543 amino acids of the carboxyl portion of the myosin heavy chain. The DNA sequence and encoded amino acid sequence were compared to the hamster alpha (pVHC1) and beta (pVHC2/pVHC3) cardiac myosin heavy chain cDNA and amino acid sequences and the rat cardiac myosin heavy chain sequences as well. The myosin heavy chain mRNAs are highly conserved and this is reflected in our cDNA clone. The pHMC3 clone is 87.9% homologous to the hamster alpha cDNA and 92.2% homologous to the hamster beta cDNA clones. The 3' untranslated region of pHMC3 is 64.1% homologous to the hamster beta clone while the hamster alpha myosin heavy chain shows only 25% homology to pHMC3 and exhibits extensive diversity. Similar results were obtained when pHMC3 was compared to the rat cardiac myosin heavy chain cDNA sequences. The comparisons showed that pHMC3 is a beta cardiac myosin heavy chain cDNA clone.

PubMed Disclaimer

Similar articles

• Two different forms of beta myosin heavy chain are expressed in human striated muscle.
  Jandreski MA, Sole MJ, Liew CC. Jandreski MA, et al. Hum Genet. 1987 Oct;77(2):127-31. doi: 10.1007/BF00272378. Hum Genet. 1987. PMID: 3653886
• Construction and characterization of the alpha form of a cardiac myosin heavy chain cDNA clone and its developmental expression in the Syrian hamster.
  Liew CC, Jandreski MA. Liew CC, et al. Proc Natl Acad Sci U S A. 1986 May;83(10):3175-9. doi: 10.1073/pnas.83.10.3175. Proc Natl Acad Sci U S A. 1986. PMID: 3458174 Free PMC article.
• Characterization of genomic clones specifying rabbit alpha- and beta-ventricular myosin heavy chains.
  Friedman DJ, Umeda PK, Sinha AM, Hsu HJ, Jakovcic S, Rabinowitz M. Friedman DJ, et al. Proc Natl Acad Sci U S A. 1984 May;81(10):3044-8. doi: 10.1073/pnas.81.10.3044. Proc Natl Acad Sci U S A. 1984. PMID: 6328491 Free PMC article.
• Isolation and characterization of a previously unrecognized myosin heavy chain gene present in the Syrian hamster.
  McCully JD, Wang RX, Kellam B, Sole MJ, Liew CC. McCully JD, et al. J Mol Biol. 1991 Apr 20;218(4):657-65. doi: 10.1016/0022-2836(91)90251-z. J Mol Biol. 1991. PMID: 2023240 Review.
• Molecular adaptation to pressure overload in human and rat hearts.
  Yazaki Y, Tsuchimochi H, Kurabayashi M, Komuro I. Yazaki Y, et al. J Mol Cell Cardiol. 1989 Dec;21 Suppl 5:91-101. doi: 10.1016/0022-2828(89)90775-x. J Mol Cell Cardiol. 1989. PMID: 2534142 Review.

Cited by

• Complete sequence and organization of the human cardiac beta-myosin heavy chain gene.
  Liew CC, Sole MJ, Yamauchi-Takihara K, Kellam B, Anderson DH, Lin LP, Liew JC. Liew CC, et al. Nucleic Acids Res. 1990 Jun 25;18(12):3647-51. doi: 10.1093/nar/18.12.3647. Nucleic Acids Res. 1990. PMID: 2362820 Free PMC article. No abstract available.
• Isolation and localization of the skeletal myosin heavy chain 2X gene on pig chromosome 12q1.4-q1.5.
  Zijlstra C, Davoli R, Fontanesi L, Zambonelli P, Bosma AA, Russo V. Zijlstra C, et al. Mamm Genome. 1998 May;9(5):412-3. doi: 10.1007/s003359900785. Mamm Genome. 1998. PMID: 9545507 No abstract available.
• Thyroid hormone regulates expression of a transfected human alpha-myosin heavy-chain fusion gene in fetal rat heart cells.
  Tsika RW, Bahl JJ, Leinwand LA, Morkin E. Tsika RW, et al. Proc Natl Acad Sci U S A. 1990 Jan;87(1):379-83. doi: 10.1073/pnas.87.1.379. Proc Natl Acad Sci U S A. 1990. PMID: 2296592 Free PMC article.
• Isolation and characterization of the complete human beta-myosin heavy chain gene.
  Diederich KW, Eisele I, Ried T, Jaenicke T, Lichter P, Vosberg HP. Diederich KW, et al. Hum Genet. 1989 Feb;81(3):214-20. doi: 10.1007/BF00278991. Hum Genet. 1989. PMID: 2522082

References

1. 1. Mol Cell Biol. 1982 Feb;2(2):161-70 - PubMed
2. 1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed
3. 1. Mol Cell Biol. 1984 May;4(5):956-65 - PubMed
4. 1. J Clin Invest. 1982 Apr;69(4):816-25 - PubMed
5. 1. Nucleic Acids Res. 1982 Oct 25;10(20):6451-63 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • GlyGen glycoinformatics resource