Varicella-zoster virus specifies a thymidylate synthetase - PubMed (original) (raw)
Comparative Study
Varicella-zoster virus specifies a thymidylate synthetase
R Thompson et al. J Gen Virol. 1987 May.
Abstract
A homology search of proteins predicted from the recently reported complete DNA sequence of varicella-zoster virus (VZV) revealed that the product of gene 13 was highly homologous to eukaryotic and prokaryotic thymidylate synthetases (TSs). The VZV protein was shown to be a TS by three functional tests. Firstly, a plasmid designed to express the native protein was able to complement a strain of Escherichia coli in which the natural TS gene is deleted. Secondly, in an enzyme assay for TS, extracts of the complemented strain were capable of releasing tritiated water from 2'-deoxy[5-3H]uridylate. Thirdly, these extracts contained a protein that bound isotopically labelled 5-fluoro-2'-deoxyuridylate, a ligand specific for the active site of TS. In addition, a novel ligand-binding protein was detected in human cells infected with VZV.
Similar articles
- Human herpesvirus 8 gene encodes a functional thymidylate synthase.
Gáspár G, De Clercq E, Neyts J. Gáspár G, et al. J Virol. 2002 Oct;76(20):10530-2. doi: 10.1128/jvi.76.20.10530-10532.2002. J Virol. 2002. PMID: 12239332 Free PMC article. - Generation of varicella-zoster virus (VZV) and viral mutants from cosmid DNAs: VZV thymidylate synthetase is not essential for replication in vitro.
Cohen JI, Seidel KE. Cohen JI, et al. Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7376-80. doi: 10.1073/pnas.90.15.7376. Proc Natl Acad Sci U S A. 1993. PMID: 8394020 Free PMC article. - Characterization of the proteinase specified by varicella-zoster virus gene 33.
McMillan DJ, Kay J, Mills JS. McMillan DJ, et al. J Gen Virol. 1997 Sep;78 ( Pt 9):2153-7. doi: 10.1099/0022-1317-78-9-2153. J Gen Virol. 1997. PMID: 9292001 - Site-directed mutagenesis of varicella-zoster virus thymidylate synthase. Analysis of two highly conserved regions of the enzyme.
Harrison PT, Scott JE, Hutchinson MJ, Thompson R. Harrison PT, et al. Eur J Biochem. 1995 Jun 1;230(2):511-6. doi: 10.1111/j.1432-1033.1995.0511h.x. Eur J Biochem. 1995. PMID: 7607223
Cited by
- Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase.
Robertson GR, Whalley JM. Robertson GR, et al. Nucleic Acids Res. 1988 Dec 9;16(23):11303-17. doi: 10.1093/nar/16.23.11303. Nucleic Acids Res. 1988. PMID: 2849761 Free PMC article. - Human herpesvirus 8 open reading frame 21 is a thymidine and thymidylate kinase of narrow substrate specificity that efficiently phosphorylates zidovudine but not ganciclovir.
Gustafson EA, Schinazi RF, Fingeroth JD. Gustafson EA, et al. J Virol. 2000 Jan;74(2):684-92. doi: 10.1128/jvi.74.2.684-692.2000. J Virol. 2000. PMID: 10623730 Free PMC article. - Isolation of peptide aptamers that inhibit intracellular processes.
Blum JH, Dove SL, Hochschild A, Mekalanos JJ. Blum JH, et al. Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2241-6. doi: 10.1073/pnas.040573397. Proc Natl Acad Sci U S A. 2000. PMID: 10688899 Free PMC article. - Human herpesvirus 8 gene encodes a functional thymidylate synthase.
Gáspár G, De Clercq E, Neyts J. Gáspár G, et al. J Virol. 2002 Oct;76(20):10530-2. doi: 10.1128/jvi.76.20.10530-10532.2002. J Virol. 2002. PMID: 12239332 Free PMC article. - Structure of the Varicella Zoster Virus Thymidylate Synthase Establishes Functional and Structural Similarities as the Human Enzyme and Potentiates Itself as a Target of Brivudine.
Hew K, Dahlroth SL, Veerappan S, Pan LX, Cornvik T, Nordlund P. Hew K, et al. PLoS One. 2015 Dec 2;10(12):e0143947. doi: 10.1371/journal.pone.0143947. eCollection 2015. PLoS One. 2015. PMID: 26630264 Free PMC article.