Epstein-Barr virus nuclear antigen 2 specifically induces expression of the B-cell activation antigen CD23 - PubMed (original) (raw)

Epstein-Barr virus nuclear antigen 2 specifically induces expression of the B-cell activation antigen CD23

F Wang et al. Proc Natl Acad Sci U S A. 1987 May.

Abstract

Epstein-Barr virus (EBV) infection of EBV-negative Burkitt lymphoma (BL) cells induces some changes similar to those seen in normal B lymphocytes that have been growth transformed by EBV. The role of individual EBV genes in this process was evaluated by introducing each of the viral genes that are normally expressed in EBV growth-transformed and latently infected lymphoblasts into an EBV-negative BL cell line, using recombinant retrovirus-mediated transfer. Clones of cells were derived that stably express the EBV nuclear antigen 1 (EBNA-1), EBNA-2, EBNA-3, EBNA-leader protein, or EBV latent membrane protein (LMP). These were compared with control clones infected with the retrovirus vector. All 10 clones converted to EBNA-2 expression differed from control clones or clones expressing other EBV proteins by growth in tight clumps and by markedly increased expression of one particular surface marker of B-cell activation, CD23. Other activation antigens were unaffected by EBNA-2 expression, as were markers already expressed on the parent BL cell line, including BL markers (cALLA and BLA), proliferation markers (transferrin receptor and BK19.9), and cell adhesion-related molecules (LFA-1 and LFA-3). Increased CD23 expression in cells expressing EBNA-2 was apparent from monoclonal anti-CD23 antibody binding to the cell surface, from immunoprecipitation of the 45-kDa and 90-kDa CD23 proteins with monoclonal antibody, and from RNA blots probed with labeled CD23 DNA. The results indicate that EBNA-2 is a specific direct or indirect trans-activator of CD23. This establishes a link between an EBV gene and cell gene expression. Since CD23 has been implicated in the transduction of B-cell growth signals, its specific induction by EBNA-2 could be important in EBV induction of B-lymphocyte transformation.

PubMed Disclaimer

Similar articles

• Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are effectors of phenotypic changes in B lymphocytes: EBNA-2 and LMP1 cooperatively induce CD23.
  Wang F, Gregory C, Sample C, Rowe M, Liebowitz D, Murray R, Rickinson A, Kieff E. Wang F, et al. J Virol. 1990 May;64(5):2309-18. doi: 10.1128/JVI.64.5.2309-2318.1990. J Virol. 1990. PMID: 2157887 Free PMC article.
• Stable transfection of Epstein-Barr virus (EBV) nuclear antigen 2 in lymphoma cells containing the EBV P3HR1 genome induces expression of B-cell activation molecules CD21 and CD23.
  Cordier M, Calender A, Billaud M, Zimber U, Rousselet G, Pavlish O, Banchereau J, Tursz T, Bornkamm G, Lenoir GM. Cordier M, et al. J Virol. 1990 Mar;64(3):1002-13. doi: 10.1128/JVI.64.3.1002-1013.1990. J Virol. 1990. PMID: 2154588 Free PMC article.
• T cell recognition of Epstein-Barr virus associated lymphomas.
  Rickinson AB, Murray RJ, Brooks J, Griffin H, Moss DJ, Masucci MG. Rickinson AB, et al. Cancer Surv. 1992;13:53-80. Cancer Surv. 1992. PMID: 1330300 Review.
• Immortalizing genes of Epstein-Barr virus.
  Middleton T, Gahn TA, Martin JM, Sugden B. Middleton T, et al. Adv Virus Res. 1991;40:19-55. doi: 10.1016/s0065-3527(08)60276-6. Adv Virus Res. 1991. PMID: 1659776 Review.

Cited by

• Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids.
  Contreras-Salazar B, Klein G, Masucci MG. Contreras-Salazar B, et al. J Virol. 1989 Jun;63(6):2768-72. doi: 10.1128/JVI.63.6.2768-2772.1989. J Virol. 1989. PMID: 2542588 Free PMC article.
• Biochemical characterization of Epstein-Barr virus nuclear antigen 2A.
  Grässer FA, Haiss P, Göttel S, Mueller-Lantzsch N. Grässer FA, et al. J Virol. 1991 Jul;65(7):3779-88. doi: 10.1128/JVI.65.7.3779-3788.1991. J Virol. 1991. PMID: 1645792 Free PMC article.
• A sixth Epstein-Barr virus nuclear protein (EBNA3B) is expressed in latently infected growth-transformed lymphocytes.
  Petti L, Kieff E. Petti L, et al. J Virol. 1988 Jun;62(6):2173-8. doi: 10.1128/JVI.62.6.2173-2178.1988. J Virol. 1988. PMID: 2835517 Free PMC article.
• Epstein-Barr virus nuclear protein EBNA3C is required for cell cycle progression and growth maintenance of lymphoblastoid cells.
  Maruo S, Wu Y, Ishikawa S, Kanda T, Iwakiri D, Takada K. Maruo S, et al. Proc Natl Acad Sci U S A. 2006 Dec 19;103(51):19500-5. doi: 10.1073/pnas.0604919104. Epub 2006 Dec 11. Proc Natl Acad Sci U S A. 2006. PMID: 17159137 Free PMC article.
• Cellular target genes of Epstein-Barr virus nuclear antigen 2.
  Maier S, Staffler G, Hartmann A, Höck J, Henning K, Grabusic K, Mailhammer R, Hoffmann R, Wilmanns M, Lang R, Mages J, Kempkes B. Maier S, et al. J Virol. 2006 Oct;80(19):9761-71. doi: 10.1128/JVI.00665-06. J Virol. 2006. PMID: 16973580 Free PMC article.

References

1. 1. Science. 1967 Sep 1;157(3792):1064-5 - PubMed
2. 1. Int J Cancer. 1968 Nov 15;3(6):857-66 - PubMed
3. 1. Nature. 1971 May 7;231(5297):46-7 - PubMed
4. 1. Nature. 1977 May 5;267(5606):52-4 - PubMed
5. 1. Eur J Cancer. 1977 Nov;13(11):1269-75 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database