Desorption of porcine parvovirus from aluminum hydroxide adjuvant with subsequent viral immunoassay or hemagglutination assay - PubMed (original) (raw)

• PMID: 3033874
• DOI: 10.1007/BF00361329

Desorption of porcine parvovirus from aluminum hydroxide adjuvant with subsequent viral immunoassay or hemagglutination assay

J Katz. Vet Res Commun. 1987.

Abstract

Cell culture fluids containing porcine parvovirus were adjuvanted with varying concentrations of aluminum hydroxide gel. Adsorption of virus and total protein to adjuvant was proportional to adjuvant concentration. Desorption of virus and protein from the adjuvant in substantial, reproducible quantities was achieved by washing adjuvanted preparations with 1.2 M potassium phosphate, followed by dialysis and concentration of wash fluids. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of unadjuvanted viral fluids, supernatants of adjuvanted mixtures, and desorptive washings of the corresponding adjuvant pellets revealed no qualitative differences in protein banding patterns. Desorbed virus was quantitated by a hemagglutination assay, or by an enzyme-linked immunoassay employing a monoclonal anti-porcine parvovirus antibody. Virus desorption and subsequent assays permitted in vitro estimation of virus content in adjuvanted porcine parvovirus preparations. This approach may be useful in estimating the antigen content of inactivated aluminum hydroxide adjuvanted veterinary vaccines and reducing the extent of required in vivo testing.

PubMed Disclaimer

Similar articles

• In vitro assessment of viral antigen content in inactivated aluminum hydroxide adjuvanted vaccines.
  Katz JB, Hanson SK, Patterson PA, Stoll IR. Katz JB, et al. J Virol Methods. 1989 Jul;25(1):101-8. doi: 10.1016/0166-0934(89)90104-3. J Virol Methods. 1989. PMID: 2550498
• Radioimmunoassay of adjuvant-associated porcine parvovirus using a monoclonal antibody in a nitrocellulose membrane system.
  Katz JB, van Deusen RA. Katz JB, et al. J Virol Methods. 1985 Dec;12(3-4):193-8. doi: 10.1016/0166-0934(85)90129-6. J Virol Methods. 1985. PMID: 3009510
• Potentiating effect of adjuvants on humoral immunity to porcine parvovirus vaccines in guinea pigs.
  Molitor TW, Joo HS, Thacker BJ. Molitor TW, et al. Vet Microbiol. 1985 Apr;10(3):209-18. doi: 10.1016/0378-1135(85)90047-1. Vet Microbiol. 1985. PMID: 4002607
• Detection of antibodies against porcine parvovirus in swine sera by enzyme-linked immunosorbent assay.
  Hohdatsu T, Baba K, Ide S, Tsuchimoto M, Nagano H, Yamagami T, Yamagishi H, Fujisaki Y, Matumoto M. Hohdatsu T, et al. Vet Microbiol. 1988 May;17(1):11-9. doi: 10.1016/0378-1135(88)90075-2. Vet Microbiol. 1988. PMID: 2845632
• Efficacy of porcine parvovirus vaccines.
  Edwards KR, Emmerson MA, Luff PR, Wells DE, Muskett JC, Wrathall AE, Richardson C, Parker BN, Thornton DH. Edwards KR, et al. Vet Rec. 1986 Aug 30;119(9):203-6. doi: 10.1136/vr.119.9.203. Vet Rec. 1986. PMID: 3020765

Cited by

• An electrochemiluminescence assay for analysis of rabies virus glycoprotein content in rabies vaccines.
  Smith TG, Ellison JA, Ma X, Kuzmina N, Carson WC, Rupprecht CE. Smith TG, et al. Vaccine. 2013 Jul 18;31(33):3333-8. doi: 10.1016/j.vaccine.2013.05.081. Epub 2013 Jun 3. Vaccine. 2013. PMID: 23742991 Free PMC article.
• The importance of surface charge in the optimization of antigen-adjuvant interactions.
  Callahan PM, Shorter AL, Hem SL. Callahan PM, et al. Pharm Res. 1991 Jul;8(7):851-8. doi: 10.1023/a:1015843210358. Pharm Res. 1991. PMID: 1924135
• Development of a Direct Alhydrogel Formulation Immunoassay (DAFIA).
  Zhu D, Huang S, Gebregeorgis E, McClellan H, Dai W, Miller L, Saul A. Zhu D, et al. J Immunol Methods. 2009 May 15;344(1):73-8. doi: 10.1016/j.jim.2009.03.005. Epub 2009 Mar 27. J Immunol Methods. 2009. PMID: 19328804 Free PMC article.
• Use of o-phthalaldehyde assay to determine protein contents of Alhydrogel-based vaccines.
  Zhu D, Saul A, Huang S, Martin LB, Miller LH, Rausch KM. Zhu D, et al. Vaccine. 2009 Oct 9;27(43):6054-9. doi: 10.1016/j.vaccine.2009.07.067. Epub 2009 Aug 4. Vaccine. 2009. PMID: 19660590 Free PMC article.
• Working together: interactions between vaccine antigens and adjuvants.
  Fox CB, Kramer RM, Barnes V L, Dowling QM, Vedvick TS. Fox CB, et al. Ther Adv Vaccines. 2013 May;1(1):7-20. doi: 10.1177/2051013613480144. Ther Adv Vaccines. 2013. PMID: 24757512 Free PMC article.

References

1. 1. Anal Biochem. 1978 Jun 15;87(1):206-10 - PubMed
2. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
3. 1. J Biol Stand. 1982 Jul;10(3):185-95 - PubMed
4. 1. J Immunol. 1962 Mar;88:320-9 - PubMed
5. 1. Zentralbl Bakteriol Orig. 1959 Apr;174(7-8):473-92 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database