Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse - PubMed (original) (raw)

Comparative Study

Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse

D R Gewert et al. EMBO J. 1987 Mar.

Abstract

A genomic clone encompassing the entire coding region of a murine gene homologous to human erythroid potentiating activity/tissue inhibitor of metalloproteinase (EPA/TIMP) was isolated and sequenced. Based on alignment with human EPA/TIMP cDNAs we deduce a structure comprising five exons and four introns extending over 4.3 kb of DNA. In mouse and hamster cell lines transcription from this gene and interferon genes is induced by Newcastle Disease virus (NDV). Examination of the 5'-flanking sequences of the gene reveals a set of repeated elements with structural similarity to those previously described as inducer-responsive elements in the human IFN-beta 1 gene. The 4.3-kb DNA fragment encompassing the homologous murine EPA/TIMP gene was transfected into human T98G cells and transfectants tested for NDV inducibility. In contrast to the endogenous human gene, the integrated murine EPA/TIMP gene was NDV-inducible and TIMP activity was detectable in the cell culture fluid.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Science. 1977 Apr 8;196(4286):180-2 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Eur J Biochem. 1979 Jul;98(1):215-21 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 - PubMed
    1. Anal Biochem. 1979 Nov 1;99(2):340-5 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources