Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse - PubMed (original) (raw)
Comparative Study
Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mouse
D R Gewert et al. EMBO J. 1987 Mar.
Abstract
A genomic clone encompassing the entire coding region of a murine gene homologous to human erythroid potentiating activity/tissue inhibitor of metalloproteinase (EPA/TIMP) was isolated and sequenced. Based on alignment with human EPA/TIMP cDNAs we deduce a structure comprising five exons and four introns extending over 4.3 kb of DNA. In mouse and hamster cell lines transcription from this gene and interferon genes is induced by Newcastle Disease virus (NDV). Examination of the 5'-flanking sequences of the gene reveals a set of repeated elements with structural similarity to those previously described as inducer-responsive elements in the human IFN-beta 1 gene. The 4.3-kb DNA fragment encompassing the homologous murine EPA/TIMP gene was transfected into human T98G cells and transfectants tested for NDV inducibility. In contrast to the endogenous human gene, the integrated murine EPA/TIMP gene was NDV-inducible and TIMP activity was detectable in the cell culture fluid.
Similar articles
- Presence of transcription regulatory elements within an intron of the virus-inducible murine TIMP gene.
Coulombe B, Ponton A, Daigneault L, Williams BR, Skup D. Coulombe B, et al. Mol Cell Biol. 1988 Aug;8(8):3227-34. doi: 10.1128/mcb.8.8.3227-3234.1988. Mol Cell Biol. 1988. PMID: 2850484 Free PMC article. - Transcriptional activity of the human tissue inhibitor of metalloproteinases 1 (TIMP-1) gene in fibroblasts involves elements in the promoter, exon 1 and intron 1.
Clark IM, Rowan AD, Edwards DR, Bech-Hansen T, Mann DA, Bahr MJ, Cawston TE. Clark IM, et al. Biochem J. 1997 Jun 1;324 ( Pt 2)(Pt 2):611-7. doi: 10.1042/bj3240611. Biochem J. 1997. PMID: 9182725 Free PMC article. - Murine tissue inhibitor of metalloproteinases-4 (Timp-4): cDNA isolation and expression in adult mouse tissues.
Leco KJ, Apte SS, Taniguchi GT, Hawkes SP, Khokha R, Schultz GA, Edwards DR. Leco KJ, et al. FEBS Lett. 1997 Jan 20;401(2-3):213-7. doi: 10.1016/s0014-5793(96)01474-3. FEBS Lett. 1997. PMID: 9013889
Cited by
- MMPs and TIMPs--an historical perspective.
Woessner JF Jr. Woessner JF Jr. Mol Biotechnol. 2002 Sep;22(1):33-49. doi: 10.1385/MB:22:1:033. Mol Biotechnol. 2002. PMID: 12353913 Review. - Stromelysin-1 regulates adipogenesis during mammary gland involution.
Alexander CM, Selvarajan S, Mudgett J, Werb Z. Alexander CM, et al. J Cell Biol. 2001 Feb 19;152(4):693-703. doi: 10.1083/jcb.152.4.693. J Cell Biol. 2001. PMID: 11266461 Free PMC article. - Gelatinase B is required for alveolar bronchiolization after intratracheal bleomycin.
Betsuyaku T, Fukuda Y, Parks WC, Shipley JM, Senior RM. Betsuyaku T, et al. Am J Pathol. 2000 Aug;157(2):525-35. doi: 10.1016/S0002-9440(10)64563-4. Am J Pathol. 2000. PMID: 10934155 Free PMC article. - A molecular strategy designed for the rapid screening of gene traps based on sequence identity and gene expression pattern in adult mice.
Holzschu D, Lapierre L, Neubaum D, Mark WH. Holzschu D, et al. Transgenic Res. 1997 Jan;6(1):97-106. doi: 10.1023/a:1018465402294. Transgenic Res. 1997. PMID: 9032982 - Expression of collagen, interstitial collagenase, and tissue inhibitor of metalloproteinases-1 in restenosis after carotid endarterectomy.
Nikkari ST, Geary RL, Hatsukami T, Ferguson M, Forough R, Alpers CE, Clowes AW. Nikkari ST, et al. Am J Pathol. 1996 Mar;148(3):777-83. Am J Pathol. 1996. PMID: 8774133 Free PMC article.
References
- Science. 1977 Apr 8;196(4286):180-2 - PubMed
- J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
- Eur J Biochem. 1979 Jul;98(1):215-21 - PubMed
- Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 - PubMed
- Anal Biochem. 1979 Nov 1;99(2):340-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
Research Materials
Miscellaneous